Abstract: [Objective and Metod]In this experiment,the callus regeneration system of Lavender was established by the use of it as meterial,which layed the basis for fast reproduction of Lavender and genetic transformation.[Result]It was shown that the induction results of the MS +2,4-D 0.10 mg/L + KT0. 30 mg/L medium groups were better than that of other groups.The yellowish callus from 2-3 generations were continuously cultivated on the medium of MS+6-BA 2.0 mg/L+NAA 0.50 mg/L,which form great number of thickly-growing root,when young bud reached 1-2 cm,then was put in medium with MS+6-BA 1.0 mg/L to be expanded for 1-2 generations.After that,the young bud was put in medium with 1/2 MS+IAA 1.0 mg/L to continue to be cultiveated to be rooting.[Conclusion]In 20 days,the rooting rate reached 33;.