无核白葡萄芽变系的植物学性状特征及RAPD标记研究

无核白葡萄芽变系的植物学性状特征及RAPD标记研究

闫鹏;骆强伟;廖康;孙锋

新疆农业大学林学与园艺学院,乌鲁木齐,830091;新疆葡萄瓜果开发研究中心,新疆鄯善,838200

收稿日期:2010-01-25 修回日期:2010-01-25 出版日期:2010-01-25 发布日期:2010-01-25

Research on Botanical Characteristers and RAPD Marker of Thomson Seedless Bud Variation

YAN Peng;LUO Qiang-wei;LIAO Kang;SUN Feng

Received:2010-01-25 Revised:2010-01-25 Online:2010-01-25 Published:2010-01-25

摘要/Abstract

摘要： [目的]了解芽变植株的植物学特性及遗传背景.[方法]以普通无核白做对照,对其10个芽变株系的生物学和植物学特性进行观测,并对其幼叶进行RAPD分析.[结果]芽变株系在植物学特征上存在不同程度的变异,主要体现在果实性状上.[结论]初步发现1株4倍体芽变.通过RAPD分析,采用470个10碱基随机引物进行RAPD多态性检测,共筛选出能产生多态性标记的RAPD引物3个.

Abstract: [Objective and Metnod]The study was carried out to observe the botanical and biological characters of thomson seedless bud variation and analyze young leaves by RAPD,using thomson seedlings as the control.[Result]There were different variations in bud variation of botanical characters.It was found that there was a bud variation with tetraploid in the test.[Conclusion]Through RAPD analysis, by using 470 random 10-alkali primers, RAPD polymorphism were detected from 30 samples. The polymorphism information could be provided with 3 random primers.

闫鹏;骆强伟;廖康;孙锋. 无核白葡萄芽变系的植物学性状特征及RAPD标记研究[J]. .

YAN Peng;LUO Qiang-wei;LIAO Kang;SUN Feng. Research on Botanical Characteristers and RAPD Marker of Thomson Seedless Bud Variation[J]. .