Abstract: [Objective and Method]Ten endophytes were isolated from yam rhizome, using five different isolation media. Four microbes were chosen for 16S rRNA gene sequence and systematic analysis.[Result]Results of 16S rRNA gene sequences blasting showed that the strains were within Erwinia genus and Bacillus genus, no actinomycete and fungus was found, which indicated that there was a poor diversity of endophytes in yam rhizome. The max homology of 16S rRNA of the produced polysaccharide strain SS2 product from yam rhizome leaching solution and model strain Erwinia pyrifoliae Ep1/96T was 97.1;.[Conclusion]which suggested the strain may be a novel species.