新疆农业科学 ›› 2024, Vol. 61 ›› Issue (1): 118-126.DOI: 10.6048/j.issn.1001-4330.2024.01.013
姚军1,2(), 秦勇2(), 郑贺云1, 张翠环1, 再吐娜·买买提1, 汪志伟1, 耿新丽1()
收稿日期:
2023-05-09
出版日期:
2024-01-20
发布日期:
2024-02-21
通信作者:
耿新丽(1975-),女,河南人,研究员,硕士,研究方向为农产品贮藏与保鲜,(E-mail)作者简介:
姚军(1978-),男,四川人,副研究员,硕士,研究方向为农产品贮藏与保鲜,(E-mail)53260292@qq.com
基金资助:
YAO Jun1,2(), QIN Yong2(), ZHENG Heyun1, ZHANG Cuihuan1, Zaituna Maimaiti1, WANG Zhiwei1, GEN Xinli1()
Received:
2023-05-09
Online:
2024-01-20
Published:
2024-02-21
Correspondence author:
GENG Xinlin(1975-),female,Xinjinag,research,research field:storage and preservation of agricultural products,(E-mail)Supported by:
摘要:
【目的】研究一种无毒安全、绿色环保、廉价易得的植物源抑菌材料,并在甜瓜贮藏与保鲜中应用,为无花果叶提取液复合保鲜膜在甜瓜上的应用提供参考。【方法】优化具有抑菌效果的植物源材料无花果叶提取液的提取工艺,采用响应面法优化乙醇浓度、超声时间、料液比并分析对无花果叶提取液总黄酮取得率的影响。采用十字交叉法测定提取液对甜瓜采后主要病原菌镰刀菌、链格孢的抑菌效果,并将无花果叶提取剂与被膜剂海藻酸钠,抗氧化剂柠檬酸,助剂蔗糖酯进行复合,采用正交法优化各制剂对贮藏期甜瓜腐烂率和失重率的影响。【结果】获得无花果叶提取液的最佳提取工艺参数:乙醇体积百分比为79.73%、超声时间为36.81 min、料液比为1∶24。无花果叶提取液复合膜保鲜剂的最佳组成配方为80 mg/mL无花果提取液+1.5%海藻酸钠+500 mg/L柠檬酸+0.5%蔗糖酯。【结论】无花果叶提取液在抑制甜瓜采后主要病原真菌上效果显著,无花果叶提取液复合保鲜膜在减少甜瓜贮藏期腐烂率和失重率降低上效果明显,可作为甜瓜贮藏保鲜的新型绿色的植物源保鲜材料运用于甜瓜生产中。
中图分类号:
姚军, 秦勇, 郑贺云, 张翠环, 再吐娜·买买提, 汪志伟, 耿新丽. 无花果叶提取液复合保鲜膜的研制及其在甜瓜保鲜上的应用[J]. 新疆农业科学, 2024, 61(1): 118-126.
YAO Jun, QIN Yong, ZHENG Heyun, ZHANG Cuihuan, Zaituna Maimaiti, WANG Zhiwei, GEN Xinli. Development of composite plastic film of extract from Ficus carica leaves and the cling film application in keeping melon fresh[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 118-126.
水平 Level | 因素Factor | |||
---|---|---|---|---|
海藻酸钠 Sodium alginate (%) | 柠檬酸 Citric acid (mg/L) | 无花果 提取液 FIG extract (mg/mL) | 蔗糖酯 Sucrose Ester (%) | |
1 | 1.0 | 300 | 20 | 0 |
2 | 1.5 | 500 | 50 | 0.5 |
3 | 2.0 | 700 | 80 | 1.0 |
表1 L9(34)3水平4因素的正交试验
Tab.1 L9(34) is the orthogonal table of 3 levels and 4 factors
水平 Level | 因素Factor | |||
---|---|---|---|---|
海藻酸钠 Sodium alginate (%) | 柠檬酸 Citric acid (mg/L) | 无花果 提取液 FIG extract (mg/mL) | 蔗糖酯 Sucrose Ester (%) | |
1 | 1.0 | 300 | 20 | 0 |
2 | 1.5 | 500 | 50 | 0.5 |
3 | 2.0 | 700 | 80 | 1.0 |
试验号 Test number | A(乙醇 浓度) Ethanol concen- tration (%) | B(超声 时间) Ultrasonic time (min) | C(料液比) Feed-to- liquid ratio (%) | Y(总黄酮 取得率) Total flavonoid yield (%) |
---|---|---|---|---|
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 | 75 75 70 75 75 75 70 70 75 70 75 75 80 80 80 75 80 | 35 40 35 30 35 40 40 30 35 35 30 35 30 35 35 35 40 | 1∶20 1∶15 1∶15 1∶15 1∶20 1∶25 1∶20 1∶20 1∶20 1∶25 1∶25 1∶20 1∶20 1∶15 1∶25 1∶20 1∶20 | 1.502 1.202 1.175 1.123 1.619 1.289 1.212 1.223 1.587 1.559 1.633 1.569 1.208 1.248 1.721 1.603 1.176 |
表2 响应面结果
Tab.2 Response surface scheme and results
试验号 Test number | A(乙醇 浓度) Ethanol concen- tration (%) | B(超声 时间) Ultrasonic time (min) | C(料液比) Feed-to- liquid ratio (%) | Y(总黄酮 取得率) Total flavonoid yield (%) |
---|---|---|---|---|
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 | 75 75 70 75 75 75 70 70 75 70 75 75 80 80 80 75 80 | 35 40 35 30 35 40 40 30 35 35 30 35 30 35 35 35 40 | 1∶20 1∶15 1∶15 1∶15 1∶20 1∶25 1∶20 1∶20 1∶20 1∶25 1∶25 1∶20 1∶20 1∶15 1∶25 1∶20 1∶20 | 1.502 1.202 1.175 1.123 1.619 1.289 1.212 1.223 1.587 1.559 1.633 1.569 1.208 1.248 1.721 1.603 1.176 |
项目 Item | 自由度 Degree of freedom | 平方和(SS) Sum of squares | 均方差(MS) Mean Square error | F值 F value | Value Prob>F | 显著性 Salience |
---|---|---|---|---|---|---|
模型Model A B C AB AC BC A2 B2 C2 残差Residuals 失拟误差Misfit error 纯误差Pure error 合误差Coincidence error | 9 1 1 1 1 1 1 1 1 1 7 3 4 16 | 0.67 4.232×10-3 0.012 0.26 1.103×10-4 1.980×10-3 0.045 0.070 0.25 1.969×10-3 0.033 0.025 8.224×10-3 0.70 | 0.074 4.232×10-3 0.012 0.26 1.103×10-4 1.980×10-3 0.045 0.070 0.25 1.969×10-3 4.723×10-3 8.278×10-3 2.056×10-3 | 15.69 0.9 2.51 55.96 0.023 0.42 9.47 14.75 52.48 0.42 4.03 | 0.000 7 0.375 4 0.157 1 0.000 1 0.882 9 0.537 9 0.017 9 0.006 4 0.000 2 0.539 1 0.105 9 | ** ** * ** ** |
表3 回归模型方差
Tab.3 Regression model variance
项目 Item | 自由度 Degree of freedom | 平方和(SS) Sum of squares | 均方差(MS) Mean Square error | F值 F value | Value Prob>F | 显著性 Salience |
---|---|---|---|---|---|---|
模型Model A B C AB AC BC A2 B2 C2 残差Residuals 失拟误差Misfit error 纯误差Pure error 合误差Coincidence error | 9 1 1 1 1 1 1 1 1 1 7 3 4 16 | 0.67 4.232×10-3 0.012 0.26 1.103×10-4 1.980×10-3 0.045 0.070 0.25 1.969×10-3 0.033 0.025 8.224×10-3 0.70 | 0.074 4.232×10-3 0.012 0.26 1.103×10-4 1.980×10-3 0.045 0.070 0.25 1.969×10-3 4.723×10-3 8.278×10-3 2.056×10-3 | 15.69 0.9 2.51 55.96 0.023 0.42 9.47 14.75 52.48 0.42 4.03 | 0.000 7 0.375 4 0.157 1 0.000 1 0.882 9 0.537 9 0.017 9 0.006 4 0.000 2 0.539 1 0.105 9 | ** ** * ** ** |
处理浓度 Treatment concentration (μg/mL) | 镰刀菌属菌落直径 Colony diameter of Fusarium(mm) | 链格孢属菌落直径 Colony diameter of Alternaria(mm) | ||
---|---|---|---|---|
无花果叶提取液 FIG extract | 阿米西达 Amistad | 无花果叶提取液 FIG extract | 阿米西达 Amistad | |
0(CK) | 50.09±0.25Aa | 53.42±0.38Aa | 32.63±0.66Aa | 32.47±0.37Aa |
0.78 | 48.81±0.17Bb | 35.30±0.53Bb | 32.09±0.21ABab | 27.10±0.35Bb |
1.56 | 48.15±0.27BCc | 33.17±0.59BCc | 31.52±0.41ABCabc | 25.73±0.09Cc |
3.12 | 47.27±0.32CDd | 32.61±1.22BCDc | 31.22±0.35ABCbcd | 24.96±0.25CDcd |
6.25 | 46.76±0.17Dd | 32.13±0.55CDcd | 30.71±0.33BCDcd | 24.73±0.45CDEd |
12.5 | 45.49±0.19Ee | 30.35±0.64DEde | 30.51±0.25BCDcd | 24.62±0.10CDEd |
25 | 43.30±0.22Ff | 30.03±0.34DEe | 30.03±0.13CDde | 24.33±0.38DEde |
50 | 37.96±0.17Gg | 28.94±0.64EFef | 29.04±0.58DEe | 23.59±0.23EFe |
100 | 33.07±0.14Hh | 27.06±0.42Ff | 27.41±0.47Ef | 22.52±0.22Ff |
表4 无花果叶提取液和阿米西达下甜瓜真菌病害镰刀菌和链格孢抑菌性变化
Tab.4 Effects of ficus carica leaf extract and Amcidar on the bacteriostasis of Fusarium and Alternaria on melon fungal diseases
处理浓度 Treatment concentration (μg/mL) | 镰刀菌属菌落直径 Colony diameter of Fusarium(mm) | 链格孢属菌落直径 Colony diameter of Alternaria(mm) | ||
---|---|---|---|---|
无花果叶提取液 FIG extract | 阿米西达 Amistad | 无花果叶提取液 FIG extract | 阿米西达 Amistad | |
0(CK) | 50.09±0.25Aa | 53.42±0.38Aa | 32.63±0.66Aa | 32.47±0.37Aa |
0.78 | 48.81±0.17Bb | 35.30±0.53Bb | 32.09±0.21ABab | 27.10±0.35Bb |
1.56 | 48.15±0.27BCc | 33.17±0.59BCc | 31.52±0.41ABCabc | 25.73±0.09Cc |
3.12 | 47.27±0.32CDd | 32.61±1.22BCDc | 31.22±0.35ABCbcd | 24.96±0.25CDcd |
6.25 | 46.76±0.17Dd | 32.13±0.55CDcd | 30.71±0.33BCDcd | 24.73±0.45CDEd |
12.5 | 45.49±0.19Ee | 30.35±0.64DEde | 30.51±0.25BCDcd | 24.62±0.10CDEd |
25 | 43.30±0.22Ff | 30.03±0.34DEe | 30.03±0.13CDde | 24.33±0.38DEde |
50 | 37.96±0.17Gg | 28.94±0.64EFef | 29.04±0.58DEe | 23.59±0.23EFe |
100 | 33.07±0.14Hh | 27.06±0.42Ff | 27.41±0.47Ef | 22.52±0.22Ff |
处理 Deal with | 均值 Mean value | 标准误差 Standard error | 95%置信 区间下限 Lower limit of confidence interval | 95%置信 区间上限 Upper limit of confidence interval |
---|---|---|---|---|
无花果提取液FIG extract | ||||
20 | 2.57 | 0.064 | 2.443 | 2.697 |
50 | 2.572 | 0.064 | 2.445 | 2.699 |
80 | 2.15 | 0.064 | 2.023 | 2.277 |
海藻酸钠Sodium alginate | ||||
1 | 2.472 | 0.064 | 2.345 | 2.599 |
1.5 | 2.489 | 0.062 | 2.365 | 2.614 |
2.0 | 2.331 | 0.065 | 2.202 | 2.46 |
柠檬酸Citric acid | ||||
300 | 2.479 | 0.064 | 2.352 | 2.606 |
500 | 2.354 | 0.064 | 2.228 | 2.481 |
700 | 2.459 | 0.064 | 2.332 | 2.585 |
蔗糖酯Sucrose Ester | ||||
0 | 2.426 | 0.64 | 2.299 | 2.552 |
0.5 | 2.37 | 0.64 | 2.244 | 2.497 |
1.0 | 2.496 | 0.64 | 2.369 | 2.622 |
表5 单因素统计(失重率)
Tab.5 Single factor statistics (weight loss rate)
处理 Deal with | 均值 Mean value | 标准误差 Standard error | 95%置信 区间下限 Lower limit of confidence interval | 95%置信 区间上限 Upper limit of confidence interval |
---|---|---|---|---|
无花果提取液FIG extract | ||||
20 | 2.57 | 0.064 | 2.443 | 2.697 |
50 | 2.572 | 0.064 | 2.445 | 2.699 |
80 | 2.15 | 0.064 | 2.023 | 2.277 |
海藻酸钠Sodium alginate | ||||
1 | 2.472 | 0.064 | 2.345 | 2.599 |
1.5 | 2.489 | 0.062 | 2.365 | 2.614 |
2.0 | 2.331 | 0.065 | 2.202 | 2.46 |
柠檬酸Citric acid | ||||
300 | 2.479 | 0.064 | 2.352 | 2.606 |
500 | 2.354 | 0.064 | 2.228 | 2.481 |
700 | 2.459 | 0.064 | 2.332 | 2.585 |
蔗糖酯Sucrose Ester | ||||
0 | 2.426 | 0.64 | 2.299 | 2.552 |
0.5 | 2.37 | 0.64 | 2.244 | 2.497 |
1.0 | 2.496 | 0.64 | 2.369 | 2.622 |
处理 Deal with | 均值 Mean value | 标准误差 Standard error | 95%置信 区间下限 Lower limit of confidence interval | 95%置信 区间上限 Upper limit of confidence interval |
---|---|---|---|---|
无花果提取液FIG extract | ||||
20 | 35.926 | 2.675 | 30.594 | 41.258 |
50 | 25.556 | 2.675 | 20.224 | 30.888 |
80 | 19.833 | 2.678 | 14.494 | 25.173 |
海藻酸钠Sodium alginate | ||||
1 | 30.37 | 2.675 | 25.038 | 35.702 |
1.5 | 22.724 | 2.63 | 17.481 | 27.966 |
2.0 | 28.221 | 2.73 | 22.779 | 33.662 |
柠檬酸Citric acid | ||||
300 | 27.407 | 2.675 | 22.075 | 32.739 |
500 | 25.926 | 2.675 | 20.594 | 31.258 |
700 | 27.981 | 2.678 | 22.642 | 33.321 |
蔗糖酯Sucrose Ester | ||||
0 | 23.704 | 2.675 | 18.372 | 29.036 |
0.5 | 27.241 | 2.678 | 21.901 | 32.580 |
1.0 | 30.37 | 2.675 | 25.038 | 35.702 |
表6 单因素统计(腐烂率)
Tab.6 Single factor statistics (decay rate)
处理 Deal with | 均值 Mean value | 标准误差 Standard error | 95%置信 区间下限 Lower limit of confidence interval | 95%置信 区间上限 Upper limit of confidence interval |
---|---|---|---|---|
无花果提取液FIG extract | ||||
20 | 35.926 | 2.675 | 30.594 | 41.258 |
50 | 25.556 | 2.675 | 20.224 | 30.888 |
80 | 19.833 | 2.678 | 14.494 | 25.173 |
海藻酸钠Sodium alginate | ||||
1 | 30.37 | 2.675 | 25.038 | 35.702 |
1.5 | 22.724 | 2.63 | 17.481 | 27.966 |
2.0 | 28.221 | 2.73 | 22.779 | 33.662 |
柠檬酸Citric acid | ||||
300 | 27.407 | 2.675 | 22.075 | 32.739 |
500 | 25.926 | 2.675 | 20.594 | 31.258 |
700 | 27.981 | 2.678 | 22.642 | 33.321 |
蔗糖酯Sucrose Ester | ||||
0 | 23.704 | 2.675 | 18.372 | 29.036 |
0.5 | 27.241 | 2.678 | 21.901 | 32.580 |
1.0 | 30.37 | 2.675 | 25.038 | 35.702 |
试验号 Test number | 无花果提取液 FIG extract (mg/mL) | 海藻酸钠 Sodium alginate(%) | 柠檬酸 Citric acid (mg/L) | 蔗糖酯 Sucrose Ester(%) | 失重率 Weight loss rate | 腐烂率 Decay rate |
---|---|---|---|---|---|---|
1 | 1(20) | 1(1.0) | 1(300) | 1(0.0) | 1.322 | 30 |
2 | 1 | 2(1.5) | 2(500) | 2(0.5) | 1.511 | 20 |
3 | 1 | 3(2.0) | 3(700) | 3(1.0) | 1.453 | 40 |
4 | 2(50) | 1 | 2 | 3 | 1.185 | 30 |
5 | 2 | 2 | 3 | 1 | 1.718 | 40 |
6 | 2 | 3 | 1 | 2 | 2.077 | 30 |
7 | 3(80) | 1 | 3 | 2 | 1.459 | 20 |
8 | 3 | 2 | 1 | 3 | 1.456 | 10 |
9 | 3 | 3 | 2 | 1 | 1.016 | 30 |
失重率X1Weight loss rate X1 | 2.57 | 2.472 | 2.479 | 2.426 | ||
失重率X2Weight loss rate X2 | 2.572 | 2.489 | 2.354 | 2.37 | ||
失重率X3Weight loss rate X3 | 2.15 | 2.331 | 2.459 | 2.496 | ||
极差RRange R | 14.625 | 1.827 | 1.109 | 0.974 | ||
主次顺序 Primary and secondary order | A>C>B>D | |||||
优水平Optimal level | 80 | 2.0 | 500 | 0.5 | ||
优组合Optimal combination | A3B3C2D2 | |||||
腐烂率X1Decay rate X1 | 35.926 | 30.37 | 27.407 | 23.704 | ||
腐烂率X2Decay rate X2 | 25.556 | 22.724 | 25.926 | 27.241 | ||
腐烂率X3Decay rate X3 | 19.833 | 28.221 | 27.981 | 30.370 | ||
极差RRange R | 9.291 | 2.214 | 0.157 | 1.555 | ||
主次顺序 Primary and secondary order | A>B>D>C | |||||
优水平Optimal level | 80 | 1.5 | 500 | 0 | ||
优组合Optimal combination | A3B2C2D1 |
表7 甜瓜植物复合保鲜膜正交试验与极差
Tab.7 Orthogonal test results and range analysis of compound clingfilm for melon plants
试验号 Test number | 无花果提取液 FIG extract (mg/mL) | 海藻酸钠 Sodium alginate(%) | 柠檬酸 Citric acid (mg/L) | 蔗糖酯 Sucrose Ester(%) | 失重率 Weight loss rate | 腐烂率 Decay rate |
---|---|---|---|---|---|---|
1 | 1(20) | 1(1.0) | 1(300) | 1(0.0) | 1.322 | 30 |
2 | 1 | 2(1.5) | 2(500) | 2(0.5) | 1.511 | 20 |
3 | 1 | 3(2.0) | 3(700) | 3(1.0) | 1.453 | 40 |
4 | 2(50) | 1 | 2 | 3 | 1.185 | 30 |
5 | 2 | 2 | 3 | 1 | 1.718 | 40 |
6 | 2 | 3 | 1 | 2 | 2.077 | 30 |
7 | 3(80) | 1 | 3 | 2 | 1.459 | 20 |
8 | 3 | 2 | 1 | 3 | 1.456 | 10 |
9 | 3 | 3 | 2 | 1 | 1.016 | 30 |
失重率X1Weight loss rate X1 | 2.57 | 2.472 | 2.479 | 2.426 | ||
失重率X2Weight loss rate X2 | 2.572 | 2.489 | 2.354 | 2.37 | ||
失重率X3Weight loss rate X3 | 2.15 | 2.331 | 2.459 | 2.496 | ||
极差RRange R | 14.625 | 1.827 | 1.109 | 0.974 | ||
主次顺序 Primary and secondary order | A>C>B>D | |||||
优水平Optimal level | 80 | 2.0 | 500 | 0.5 | ||
优组合Optimal combination | A3B3C2D2 | |||||
腐烂率X1Decay rate X1 | 35.926 | 30.37 | 27.407 | 23.704 | ||
腐烂率X2Decay rate X2 | 25.556 | 22.724 | 25.926 | 27.241 | ||
腐烂率X3Decay rate X3 | 19.833 | 28.221 | 27.981 | 30.370 | ||
极差RRange R | 9.291 | 2.214 | 0.157 | 1.555 | ||
主次顺序 Primary and secondary order | A>B>D>C | |||||
优水平Optimal level | 80 | 1.5 | 500 | 0 | ||
优组合Optimal combination | A3B2C2D1 |
[1] | 黄丹丹. 无花果叶总黄酮的提取及降血糖作用研究[D]. 福州: 福建医科大学, 2015. |
HUANG Dandan. Study on extraction and hypoglycemic effect of total flavonoids from fig leaves[D]. Fuzhou: Fujian Medical University, 2015. | |
[2] | 张泽俊, 沙坤. 超声波辅助提取无花果叶中总黄酮工艺的研究[J]. 食品研究与开发, 2011, 32(6):14-16. |
ZHANG Zejun, SHA Kun. Study on extraction of total flavonoids from ficus carica leaves by ultrasound-assisted method[J]. Food Research and Development, 2011, 32(6):14-16. | |
[3] | 王振斌, 刘加友, 马海乐. 无花果多糖提取工艺优化及其超声波改性[J]. 农业工程学报, 2014, 30(10):262-269. |
WANG Zhenbin, LIU Jiayou, MA Haile. Extraction process optimization and ultrasonic modification of polysaccharide from Ficus carical[J]. Transactions of the Chinese Society of Agricultural Engineering, 2014, 30(10):262-269. | |
[4] | 张俊艳, 彭珊珊, 方园, 等. 枸杞叶、无花果叶多糖的超声波提取和测定[J]. 食品工业, 2011, 32(12):98-100. |
ZHANG Junyan, PENG Shanshan, FANG Yuan, et al. Extraction by Ultrasonic Method and Determination of Polysaccharide from Lycium barbarum Leaves and Ficus carical Leaves[J]. Food Industry, 2011, 32(12):98-100. | |
[5] |
Caro A D, Piga A. Polyphenol composition of peel and pulp of two Italian fresh fig fruits cultivars(Ficus carica L.)[J]. European Food Research and Technology, 2008, 226(4):715-719.
DOI URL |
[6] |
Veberic R, Colaric M, Stampar F. Phenolic acids and flavonoids of fig fruit(Ficus carica L.) in the northern Mediterranean region[J]. Food Chemistry, 2008, 106(1):153-157.
DOI URL |
[7] | 迪丽阿热姆·尼加提, 阿吉艾克拜尔·艾萨.HPLC法测定阿图什无花果叶汁中3种香豆素的含量[J]. 新疆医科大学学报, 2016, 39(1):52-55. |
Diliaremu Nijiati, Ajiaikebaier Aisa. Determination of three coumarins in Ficus carica L. Latex by HPLC methods[J]. Journal of Xinjiang Medical University, 2016, 39(1):52-55. | |
[8] |
Wang T, Jiao J, Gai Q Y, et al. Enhanced and Green Extraction Polyphenols and Furanocoumarins from Fig(Ficus carica L.)Leaves Using Deep Eutectic Solvents[J]. Journal of Pharmaceutical and Biomedical Analysis, 2017, 145:339-345.
DOI URL |
[9] |
Takahashi T, Okiura A, Kohno M. Phenylpropanoid Composition in Fig(Ficus carica L.) Leaves[J]. Journal of Natural Medicines, 2017, 71(4):770-775.
DOI PMID |
[10] |
Mahmoudi S, Khali M, Benkhaled A, et al. Phenolic and Flavonoid Contents,Antioxidant and Antimicrobial Activities of Leaf Extracts from Ten Algerian Ficus carica L.Varieties[J]. Asian Pacific Journal of Tropical Biomedicine, 2016, 6(3):239-245.
DOI URL |
[11] | Patil V V, PatilVR. Evaluation of Ant-inflammatory Activity of Ficus carica Linn.Leaves[J]. Indian Journal of Natural Products and Resources, 2010, 2(2):48-50. |
[12] | Shih Y Z, Huang A J, Hou C Y, et al. The Stimulating Effects of Polyphenol and Protein Fractions from Jelly Fig(Ficus awkeotsang Makino)Achenes Against Proliferation of Leukemia Cells[J]. Journal of Food & Drug Analysis, 2017, 25(4):854-861. |
[13] | 张合亮. 无花果叶提取物的制备及其抑菌效果研究[D]. 济南: 齐鲁工业大学, 2015. |
ZHANG Heliang. Study on the preparation of fig leaves extracts and their antibacterial effect[D]. Jinan: Qilu University of Technology, 2015. | |
[14] | 杜文静, 罗莉, 罗德梅, 等. 无花果叶提取物对OPG基因敲除小鼠的抗骨质疏松作用研究[J]. 中华中医药杂志, 2015, 30(10):3687-3690. |
DU Wenjing, LUO Li, LUO Demei, et al. Research on the anti-osteoporosis effect of fig leaf extraction on OPG knock out mice[J]. Chinese Journal of Traditional Chinese Medicine and Pharmacy, 2015, 30(10):3687-3690. | |
[15] | 许秋霞, 张吟, 黄丹丹, 等. 无花果叶提取物对糖尿病大鼠血糖及抗氧化能力的影响[J]. 福建医科大学学报, 2013, 47(3):146-149. |
XU Qiuxia, ZHANG Yin, HUANG Dandan, et al. Effects of ficus carica leaves extracts on blood glucose level and antioxidative ability in diabetic rats[J]. Journal of the Fujian Medical University, 2013, 47(3):146-149. | |
[16] |
张兴, 马志卿, 冯俊涛, 等. 植物源农药研究进展[J]. 中国生物防治学报, 2015, 31(5):685-698.
DOI |
ZHANG Xing, MA Zhiqing, FENG Juntao, et al. Review on research and development of botanical pesticides[J]. Chinese Journal of Biological Control, 2015, 31(5):685-698.
DOI |
|
[17] | 曾志红, 陈玲苗, 黄秋蓉. 丁香提取物对枝孢霉菌的抑菌效果及抑菌机理[J]. 森林与环境学报, 2019, 39(1):77-81. |
ZENG Zhihong, CHEN Lingmiao, HUANG Qiurong. Study on the antibacterial effect of clove extract on Cladosporium and its mechanism[J]. Journal of Forest and Environment, 2019, 39(1):77-81. | |
[18] | 谢娜, 李超, 冯俊涛, 等. 11种植物提取物对4种病原细菌的抑菌活性筛选[J]. 热带作物学报, 2018, 39(6):1166-1171. |
XIU Na, LI Chao, FENG Juntao, et al. Screening of antibacterial activities of 11 plant extracts against four pathogenic bacteria[J]. Chinese Journal of Tropical Crops, 2018, 39(6):1166-1171. | |
[19] | 曹鹏飞, 陈银华, 周慧娟, 等. 抗青枯病病菌植物杀菌剂的研究[J]. 江苏农业科学, 2017, 45(22):102-107. |
CAO Pengfei, CHEN Yinhua, ZHOU Huijuan, et al. Study on fungicides against bacterial wilt[J]. Jiangsu Agricultural Sciences, 2017, 45(22):102-107. | |
[20] | 莫熙礼, 吴彤林, 李松克, 等. 花椒提取物对薏苡黑粉病菌的抑菌机理[J]. 江苏农业科学, 2015, 43(8):131-133. |
MO Xili, WU Tonglin, LI Songke, et al. Inhibitory effects of three plant extracts on Coix lachryma-jobi smut[J]. Jiangsu Agricultural Sciences, 2015, 43(8):131-133. | |
[21] | 张厚良, 王若森, 高金辉, 等. 4种药剂对杨树烂皮病病菌的抑制作用[J]. 东北林业大学学报, 2010, 38(1):110-111. |
ZHANG Houliang, WANG Ruosen, GAO Jinhui, et al. Inhibition Effect of Four Kinds of Low toxin Insecticides to Cytospora chrysosperma[J]. Journal of Northeast Forestry University, 2010, 38(1):110-111. | |
[22] | 赵爱云, 吴神怡, 杜桂彩. 无花果叶抑菌活性成分的实验研究[J]. 青岛大学学报(自然科学版), 2005, 18(3):37-40. |
ZHAO Aiyun, WU Shenyi, DU Guicai. Experiment Study of Antibacterial Constituents of Ficus Carical Leaves[J]. Journal of Qingdao University(Natural Science Ed.), 2005, 18(3):37-40. | |
[23] | 王桂亭, 王皞, 宋艳艳, 等. 无花果叶提取物抑菌作用的实验研究[J]. 中国消毒学杂志, 2005, 22(4):374-376. |
WANG Guiting, WANG Hao, SONG Yanyan, et al. Experimental study on bacteriostatic efficacy of extract of fig leaves[J]. Chinese Journal of Disinfection, 2005, 22(4):374-376. | |
[24] | 胡丽丽, 李玉群, 金玉兰, 等. 无花果叶中抑菌活性成分—补骨脂素的研究[J]. 青岛农业大学学报(自然科学版), 2007, 24(4):264-266. |
HU Lili, LI Yuqun, JIN Yulan, et al. Studies on fungicidal constituents in ficus carica L. leaves[J]. Journal of Qingdao Agricultural University(Natural Science Ed.), 2007, 24(4):264-266. | |
[25] | 郑贺云, 耿新丽, 姚举, 等. 新疆厚皮甜瓜采后病害真菌的鉴定及多样性分析[J]. 西南农业学报, 2020, 33(4):788-796. |
ZHENG Heyun, GENG Xinli, YAO Ju, et al. Pathogen identification and their diversity of melon postharvest diseases in Xinjiang[J]. Southwest China Journal of Agricultural Sciences, 2020, 33(4):788-796. | |
[26] | 王波, 张晓艳, 黄攀, 等. 响应面法优化马齿苋黄酮超声波提取工艺[J]. 江苏农业学报, 2018, 34(1):166-171. |
WANG Bo, ZHANG Xiaoyan, HUANG Pan, et al. Optimization of ultrasonic wave extraction of flavonoid from Portulace oleracea L. by response[J]. Jiangsu Journal of Agricultural Sciences, 2018, 34(1):166-171. | |
[27] |
陈健, 孙爱东, 高雪娟. 响应面分析法优化超声波提取槟榔原花青素工艺[J]. 食品科学, 2011, 32(4):82-86.
DOI |
CHEN Jian, SUN Aidong, GAO Xuejuan. Optimization of ultrasonic extraction of Betelnut Proanthocyanidin by response surface methodology[J]. Food Science, 2011, 32(4):82-86.
DOI |
|
[28] | 徐士超, 董欢欢, 曾小静, 等. 萜类植物源农药的筛选及活性研究进展[J]. 林产化学与工业, 2019, 39(1):1-12. |
XU Shichao, DONG Huanhuan, ZENG Xiaojing, et al. Research progress in screening and bioactivity of terpenoid botanical pesticides[J]. Chemistry and Industry of Forest Products, 2019, 39(1):1-12. | |
[29] | Amri I, Hamrouni L, Hanana M, et al. Reviews on phytotoxic effects of essential oils and their individual components:News approach for weeds management[J]. International Journal of Applied Biology and Pharmaceutical Technology, 2013, 4(1) :96-114. |
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