响应面优化超声提取绿色棉纤维总黄酮

doi:10.6048/j.issn.1001-4330.2024.03.006

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (3): 576-583.DOI: 10.6048/j.issn.1001-4330.2024.03.006

• 作物遗传育种·种质资源·分子遗传学·生理生化 • 上一篇下一篇

响应面优化超声提取绿色棉纤维总黄酮

陈沛¹(), 鲍军秀¹, 王斐¹^,²^,³, 汤寿伍⁴, 刘海峰⁴(), 李鸿彬¹^,²^,³^,⁴()

1.石河子大学生命科学学院,新疆石河子 832003
2.绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子 832000
3.新疆植物药资源利用教育部重点实验室,新疆石河子 832000
4.中国彩棉(集团)股份有限公司,乌鲁木齐 830001

收稿日期:2023-08-03 出版日期:2024-03-20 发布日期:2024-04-19
通信作者: 李鸿彬(1980-),男,河南人,教授,博士,硕士生/博士生导师,研究方向为植物分子生物学,(E-mail)lihb@shzu.edu.cn;刘海峰(1977-),男,湖北人,研究员,硕士生导师,研究方向为棉花遗传育种,(E-mail)cncclhf@163.com
作者简介:陈沛(1996-),男,四川人,硕士研究生,研究方向为植物分子生物学,(E-mail)554538275@qq.com
基金资助:
新疆维吾尔自治区创新环境建设专项(2020D14035);新疆生产建设兵团科技攻关与成果转化项目(2016AC017)

Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

CHEN Pei¹(), BAO Junxiu¹, WANG Fei¹^,²^,³, TANG Shouwu⁴, LIU Haifeng⁴(), LI Hongbin¹^,²^,³^,⁴()

1. College of Life Sciences,Shihezi University,Shihezi Xinjiang 832003,China
2. Key Laboratory of Oasis Town and Mountain Basin System Ecology,Xinjiang Production and Construction Corps,Shihezi Xinjiang 832000,China
3. Key Laboratory of Xinjiang Phytomedicine Resource and Utilization,Ministry of Education,Shihezi Xinjiang 832000,China
4. China Natural Color Cotton Corp.Ltd.,Urumqi 830001,China

Received:2023-08-03 Online:2024-03-20 Published:2024-04-19
Correspondence author: LI Hongbin(1980-),male,from Henan,professor,Ph.D.,doctoral supervisor,research field:plant molecular biology,(E-mail) lihb@shzu.edu.cn;LIU Haifeng,(1977-),male,from Hubei,master supervisor,research field:cotton genetics and breeding,(E-mail) cncclhf@163.com.
Supported by:
Innovation Environment Construction Project of Xinjiang Uygur Autonomous Region(2020D14035);Tackling Hard-Nut Problems in Science and Technology and Research Results Transformation Project of XPCC(2016AC017)

摘要/Abstract

摘要：

【目的】 优化超声提取绿色棉纤维中总黄酮工艺条件,为合理利用及鉴别天然绿色棉纤维提供依据。【方法】 以天然绿色棉纤维为原料,利用超声空化效应加速黄酮溶解于乙醇中,在单因素试验基础上,结合Box-Benhnken中心组合原理及响应面设计优化试验条件。【结果】 料液比为1∶50 g/mL时,总黄酮得率达到最大值,提取最适料液比为1∶50 g/mL,响应面试验中建立的模型的CV值=0.87%,影响绿色棉纤维总黄酮得率的顺序为提取时间>乙醇浓度>料液比,A料液比为1∶50 g/mL、B乙醇浓度50%、C提取时间50 min为最佳提取条件。【结论】 超声波法提取绿色棉纤维总黄酮的最佳条件参数为料液比1∶50 g/mL、乙醇浓度50%、提取时间50 min;获得平均总黄酮得率为5.25%。

关键词: 绿色棉纤维; 总黄酮; 响应面法; 最佳提取条件; 超声波细胞破碎

Abstract:

【Objective】 This study is to optimize ultrasonic extraction conditions of total flavonoids in NGC fibers,in the hope of providing the basis for reasonable utilization and identification of NGC fibers.【Methods】 The NGC fibers were used as raw materials and were utilized for total flavonoids extract by ultrasonic cavitation effect to accelerate the dissolution of flavonoids in ethanol,to optimize the experimental conditions with Box-Benhnken center combination principle and response surface design.【Results】 The optimal extraction conditions were as follows:solid-liquid ratio of 1∶50 g/mL,ethanol concentration of 50%,and extraction time of 50 min.Under these conditions,the average yield of total flavonoids was 5.25%,which was the best condition for the extraction of total flavonoids from NGC fibers by ultrasonic method.【Conclusion】 The optimal condition Parameter for ultrasonic extraction of total flavonoids from green cotton fibers is a liquid to material ratio of 1∶50 g/mL,ethanol concentration 50%,extraction time 50 min,obtaining an average total flavonoid yield of 5.25%.

Key words: green cotton fiber; total flavonoids; response surface method; optimum extraction condition; ultrasionic cell-break

中图分类号:

S122

陈沛, 鲍军秀, 王斐, 汤寿伍, 刘海峰, 李鸿彬. 响应面优化超声提取绿色棉纤维总黄酮[J]. 新疆农业科学, 2024, 61(3): 576-583.

CHEN Pei, BAO Junxiu, WANG Fei, TANG Shouwu, LIU Haifeng, LI Hongbin. Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 576-583.

图/表 7

参考文献 24

[1]	乔建民, 安兴伦. 国内外彩色棉研究利用现状及发展前景[J]. 石河子科技, 2001,(4):19-22.
	QIAO Jianmin, AN Xinglong. Research and utilization status and development prospect of colored cotton at home and abroad[J]. Shihezi Science and Technology, 2001,(4):19-22.
[2]	Tauber M M, Guebitz G M, Rehorek A. nDegradation of Azo Dyes by Laccase and Ultrasound Treatment[J]. Applied and Environmental Microbiology, 2005, 71(5):2,600-2,607.
[3]	刘芳. 天然彩色棉与染色棉鉴别方法究[D]. 上海: 东华大学, 2008.
	LIU Fang. Study on Identification Methods of Natural Colored Cotton and Dyed Cotton[D]. Shanghai: Donghua University, 2008.
[4]	胡伯陶. 浅议天然彩色棉的色彩及其产品加工中的几个问题[J]. 棉纺织技术, 2002, 30(5):9-12.
	HU Baitao. A brief discussion on the color of natural colored cotton and some problems in product processing[J]. Cotton Textile Technology, 2002, 30(5):9-12.
[5]	张培刚, 郑鸿雁, 昌友权, 等. 松针黄酮的体外抗氧化作用研究[J]. 食品科学, 2005, 26(9):506-508.
	ZHANG Peigang, ZHENG Hongyan, CHANG Youquan, et al. Study on antioxidant activity of pine needle flavone in vitro[J]. Food Science, 2005, 26(9):506-508.
[6]	王雪, 乔博, 张健鑫, 等. 黄酮类化合物的应用研究进展[J]. 中国食品添加剂, 2020, 31(4):159-163.
	WANG Xue, QIAO Bo, ZHANG Jianxin, et al. Research progress on the application of flavonoids[J]. China Food Additives, 2020, 31(4):159-163.
[7]	马厉芳, 吴春霞, 阿不都拉·阿巴斯. 超声波提取紫草叶中总黄酮的工艺研究[J]. 食品科学, 2007, 28(10):275-277.
	MA Lifang, WU Chunxia, Abudullah Abbas. Study on ultrasonic extraction of total flavonoids from leaves of comfrey chinensis[J]. Food Science, 2007, 28(10):275-277.
[8]	王彦平, 汤高奇, 孙瑞琳, 等. 微波辅助提取葡萄皮渣总黄酮及其抗氧化性研究[J]. 食品研究与开发, 2017, 38(2):51-55.
	WANG Yanping, TANG Gaoqi, SUN Ruilin, et al. Microwave-assisted extraction of total flavonoids from grape peel residue and its antioxidant activity[J]. Food Research and Development, 2017, 38(2):51-55.
[9]	张泽生, 董晓朦, 王田心, 等. 黄秋葵中多酚和黄酮提取工艺的研究[J]. 中国食品添加剂, 2017,(1):91-99.
	ZHANG Zesheng, DONG Xiaomeng, WANG Tianxin, et al. Study on extraction technology of polyphenols and flavonoids from okra[J]. China Food Additives, 2017,(1):91-99.
[10]	王彦多, 刘春雨, 吕世洁, 等. 正交实验法优化发酵蜈蚣粉中总黄酮提取工艺[J]. 食品工业科技, 2017, 38(7):183-186,192.
	WANG Yanduo, LIU Chunyu, LYU Shijie, et al. Optimization of extraction technology of total flavonoids from fermented centipede powder by orthogonal experiment[J]. Science and Technology of Food Industry, 2017, 38(7):183-186,192.
[11]	邹维娜, 王微, 徐启江, 等. 香茶子叶片总酚、总黄酮提取及抗氧化性研究[J]. 食品工业科技, 2017, 38(2):266-272.
	ZOU Weina, WANG Wei, XU Qijiang, et al. Extraction and antioxidant activity of total phenols and flavonoids from leaves of sweet tea seed[J]. Science and Technology of Food Industry, 2017, 38(2):266-272.
[12]	闫萍, 孙海峰, 闫佳驹, 等. Box-Behnken响应面法优化翻白草总黄酮的提取工艺[J]. 中国药师, 2016, 19(12):2237-2240.
	YAN Ping, SUN Haifeng, YAN Jiaju, et al. Optimization of the extraction process of total flavonoids from rhizoma mollifolia by Box-Behnken response surface method[J]. Chinese Pharmacists, 2016, 19(12):2237-2240.
[13]	张星, 刘振春, 董欣, 等. 黑豆异黄酮超声波微波辅助提取工艺的响应面优化[J]. 西北农林科技大学学报(自然科学版), 2017, 45(6):185-192.
	ZHANG Xing, LIU Zhenchun, DONG Xin, et al. Response surface optimization of ultrasonic microwave-assisted extraction of isoflavones from black bean[J]. Journal of Northwest A & F University(Natural Science Edition), 2017, 45(6):185-192.
[14]	毕丽君, 李慧. 水芹中总黄酮类化合物最佳提取工艺的研究[J]. 食品科学, 1999, 20(12):35-37.
	BI Lijun, LI Hui. Study on the optimal extraction technology of total flavonoids from watercress[J]. Food Science, 1999, 20(12):35-37.
[15]	刘春宇, 唐丽华, 顾振纶. 不同提取方法对山楂总黄酮含量的影响[J]. 苏州医学院学报, 1998, 18(12):16-17.
	LIU Chunyu, TANG Lihua, GU Zhenlong. Effects of different extraction methods on the content of total flavonoids in hawthorn[J]. Journal of Suzhou Medical College, 1998, 18(12):16-17.
[16]	许海棠, 赵彦芝, 张金彦, 等. 响应面法提取瓶尔小草总黄酮及其抗氧化活性[J]. 食品科技, 2017, 42(2):215-220.
	XU Haitang, ZHAO Yanzhi, ZHANG Jinyan, et al. Response surface method for extraction of total flavonoids and their antioxidant activity[J]. Food Science and Technology, 2017, 42(2):215-220.
[17]	汪泽, 崔丽虹, 唐冰, 等. 响应面法优化菠萝叶纤维多酚提取工艺[J]. 食品工业科技, 2017, 38(1):219-223.
	WANG Ze, CUI Lihong, TANg Bing, et al. Optimization of polyphenol extraction from pineapple leaves by response surface method[J]. Science and Technology of Food Industry, 2017, 38(1):219-223.
[18]	邓梦琴, 林晓瑛, 张明, 等. 超声辅助提取菠萝蜜果皮黄酮工艺优化及体外抗氧化活性研究[J]. 食品工业科技, 2016, 37(24):288-293,296.
	DENG Mengqin, LIN Xiaoying, ZHANG Ming, et al. Study on extraction of flavonoids from Jackfruit by ultrasonic-assisted extraction and its antioxidant activity in vitro[J]. Science and Technology of Food Industry, 2016, 37(24):288-293,296.
[19]	张良晨, 王波, 石太渊, 等. 超声波辅助提取高粱壳黄酮色素工艺的研究[J]. 食品工业, 2016, 37(12):5-8.
	ZHANG Liangchen, WANG Bo, SHI Taiyuan, et al. Study on ultrasonic assisted extraction of flavonoid pigment from sorghum husk[J]. The Food Industry, 2016, 37(12):5-8.
[20]	王耀辉, 王景雪, 邱现创, 等. 响应面法优化白灵菇多糖超声辅助提取工艺及其体外抗氧化性[J]. 食品工业科技, 2017, 38(10):247-252.
	WANG Yaohui, WANG Jingxue, QIU Xianchuang, et al. Optimization of ultrasonic-assisted extraction process and its antioxidant activity in vitro by response surface method[J]. Science and Technology of Food Industry, 2017, 38(10):247-252.
[21]	张玉香, 屈慧鸽, 杨润亚, 等. 响应面法优化蓝莓叶黄酮的微波提取工艺[J]. 食品科学, 2010, 31(16):33-37. DOI
	ZHANG Yuxiang, QU Huige, YANG Runya, et al. Optimization of microwave extraction of flavonoids from blueberry leaves by response surface method[J]. Food Science, 2010, 31(16):33-37. DOI
[22]	庞庭才, 钟秋平, 胡上英, 等. 响应面分析法优化碱蓬黄酮提取工艺[J]. 中国酿造, 2016, 35(11):140-144.
	PANG Tingcai, ZHONG Qiuping, HU Shangying, et al. Optimization of extraction process of flavone from radix alpinae by response surface analysis[J]. China Brewing, 2016, 35(11):140-144.
[23]	谢郁峰, 陈日佳, 严春艳. 响应面法优化番石榴叶总黄酮的超声提取工艺[J]. 广东药学院学报, 2014, 30(4):417-421.
	XIE Yufeng, CHEN Rijia, YAN Chunyan. Optimization of ultrasonic extraction process of total flavonoids from guava leaves by response surface method[J]. Journal of Guangdong Pharmaceutical University, 2014, 30(4):417-421.
[24]	刘宁, 王冉, 李健, 等. 超声波细胞粉碎法提取东北红松松针总黄酮[J]. 中国食品添加剂, 2018,(4):118-127.
	LIU Ning, WANG Ran, LI Jian, et al. Extraction of total flavonoids from pine needles by ultrasonic cell crushing[J]. China Food Additives, 2018,(4):118-127.

因素 Factor	水平Level
因素 Factor	1	2	3	4	5
料液比 Solid-liquid ratio(g/mL)	1∶30	1∶40	1∶50	1∶60	1∶70
乙醇浓度 Ethanol concentration(%)	30	40	50	60	70
提取功率比 Extraction power ratio(%)	30	40	50	60	70
提取时间 Extraction time(min)	30	40	50	60	70

因素 Factor	水平Level
因素 Factor	1	2	3	4	5
料液比 Solid-liquid ratio(g/mL)	1∶30	1∶40	1∶50	1∶60	1∶70
乙醇浓度 Ethanol concentration(%)	30	40	50	60	70
提取功率比 Extraction power ratio(%)	30	40	50	60	70
提取时间 Extraction time(min)	30	40	50	60	70

试验序号 Experiment number	因素代码及编码 Factor code and coding			总黄酮得率 Total flavonoid yield(%)
试验序号 Experiment number	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentration	C.提取时间 C.Extraction time	总黄酮得率 Total flavonoid yield(%)
1	1	1	0	3.77
2	0	0	0	5.29
3	0	1	-1	4.07
4	-1	0	-1	4.01
5	1	-1	0	3.91
6	1	0	-1	4.13
7	0	0	0	5.26
8	0	-1	-1	4.02
9	0	0	0	5.21
10	0	0	0	5.27
11	1	0	1	4.13
12	0	-1	1	4.18
13	-1	-1	0	3.85
14	0	1	1	4.11
15	0	0	0	5.31
16	-1	0	1	4.15
17	-1	1	0	3.89

试验序号 Experiment number	因素代码及编码 Factor code and coding			总黄酮得率 Total flavonoid yield(%)
试验序号 Experiment number	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentration	C.提取时间 C.Extraction time	总黄酮得率 Total flavonoid yield(%)
1	1	1	0	3.77
2	0	0	0	5.29
3	0	1	-1	4.07
4	-1	0	-1	4.01
5	1	-1	0	3.91
6	1	0	-1	4.13
7	0	0	0	5.26
8	0	-1	-1	4.02
9	0	0	0	5.21
10	0	0	0	5.27
11	1	0	1	4.13
12	0	-1	1	4.18
13	-1	-1	0	3.85
14	0	1	1	4.11
15	0	0	0	5.31
16	-1	0	1	4.15
17	-1	1	0	3.89

方差来源 Source of variation	平方和 Quadratic sum	自由度 Degree of freedom	均方 Mean square	F值 F value	P值 P-value
模型Model	5.71	9	0.63	438.03	< 0.000 1
A	2.00E-04	1	2.00E-04	0.14	0.7211
B	1.80E-03	1	1.80E-03	1.24	0.301 6
C	0.014	1	0.014	9.99	0.015 9
AB	8.10E-03	1	8.10E-03	5.6	0.049 9
AC	4.90E-03	1	4.90E-03	3.39	0.108 3
BC	3.60E-03	1	3.60E-03	2.49	0.158 7
A²	2.07	1	2.07	1 431.79	< 0.000 1
B²	2.13	1	2.13	1 472.91	< 0.000 1
C²	0.9	1	0.9	619.68	< 0.000 1
失拟项Lack of fit term	4.45E-03	3	1.48E-03	1.04	0.464
误差项Error term	5.68E-03	4	1.42E-03
总和Summation	5.72	16
R²=0.998 2		R^2Adj=0.995 9		CV=0.87%

响应面优化超声提取绿色棉纤维总黄酮

Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

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参考文献 24

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Metrics

因素 Factor	编码水平Coding level
因素 Factor	-1	0	1
A.料液比 A.Solid-liquid ratio(g/mL)	1∶40	1∶50	1∶60
B.乙醇浓度 B.Ethanol concentration(%)	40	50	60
C提取时间 C:Extraction time(min)	40	50	60

项目 Items	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentr ation	C.提取时间 C.Extra ction time	总黄酮得率 Total flavonoid yield(%)
编码值 Coding value	0.000	-0.011	0.047	5.269
最佳条件 Optimum condition	1∶50 g/mL	50%	50 min	5.25

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