图1 6个Ⅰ型Gh4CL基因在棉花不同组织中的表达模式 注:A:6个Ⅰ型Gh4CL基因在棉花不同组织中的表达模式;B:6个Ⅰ型Gh4CL基因在棉花根中的相对表达量;C:6个Ⅰ型Gh4CL基因在棉花茎杆中的相对表达量

Fig.1 Expression patterns of six type I Gh4CL in different tissues of cotton Note: A:Expression patterns of six type I Gh4CLs in different tissues of cotton;B:Relative expression of six type I Gh4CL genes in cotton roots;C:Relative expression of six type I Gh4CL genes in cotton stems

图2 Gh4CL30基因沉默植株的表型和相关指标测定 注:A:TRV:00和TRV:Gh4CL30植株之间的表型差异;B:Gh4CL30基因在TRV:00和TRV:Gh4CL30植株中的相对表达量;C:TRV:00和TRV:Gh4CL30植株之间的株高和节间长度差异,由下到上依次是棉花植株的第1至4节间长度;D:TRV:00和TRV:Gh4CL30植株的木质素含量差异;E:TRV:00和TRV:Gh4CL30植株的黄酮类化合物含量差异

Fig.2 Phenotype and determination of relevant indicators in Gh4CL30 gene silenced plants Note: A:Phenotypic differences between TRV:Gh4CL30 and TRV:00 plants;B:Relative expression of Gh4CL30 in TRV:00 and TRV:Gh4CL30 plants;C:Differences in plant height and internode length between TRV:00 and TRV:Gh4CL30 plants.From bottom to top, the first to fourth internode lengths of cotton plants are shown;D:Lignin content of TRV:00 and TRV:Gh4CL30 plants;E:Flavonoid metabolite content of TRV:00 and TRV:Gh4CL30 plants

图3 Gh4CL30基因结构和编辑靶标的选择以及基因编辑类型 注:A:在Gh4CL30基因的第一个外显子区域中选择了两个编辑靶标;B:突变体的基因编辑类型

Fig.3 Gh4CL30 gene structure and selection of editing targets and types of gene editing Note:A:Two editing targets were selected in the first exon region of the Gh4CL30 gene;B:Type of gene editing of the mutant

图4 WT和4CL30植株的表型、4CL酶活性以及Gh4CL30的相对表达量 注:A:4CL30编辑植株和对照WT株高的表型比较;B:4CL30编辑植株和对照WT株高统计;C:对照WT和4CL30植株的4CL酶活性差异;D:WT和4CL30编辑植株茎秆中Gh4CL30基因相表达量

Fig.4 Phenotype, 4CL enzyme activity and relative expression of Gh4CL30 in WT and 4CL30 plants Note: A:Phenotypic comparison of 4CL30 and WT plant heights;B:Plant height statistics of 4CL30 edited plants and WT; C:The 4CL enzyme activity of WT and 4CL30 plants was different;D:Relative expression of Gh4CL30 gene in stems of WT and 4CL30 edited plants

图5 4CL30和WT植株的木质素含量以及木质素合成相关基因的表达量 注:A:使用盐酸和间苯三酚对4CL30和对照WT植物在同一节点的茎段切片染色;B:4CL30和对照WT植株的木质素含量测定;C:4CL30和对照WT植株茎秆和根部组织中木质素合成相关基因的表达量

Fig.5 Lignin content of 4CL30 and WT plants and expression of genes related to lignin synthesis Note:A:Staining of stem sections of 4CL30 and WT plants at the same node using hydrochloric acid-phloroglucinol;B:Determination of lignin content in 4CL30 and WT plants;C:Expression of genes related to lignin synthesis in stem and root tissues of 4CL30 and WT plants

图6 4CL30和对照WT植株的细胞学观察 注:A:4CL30和对照WT植物相同节茎的横向和纵向石蜡切片;B:纵向切片中的细胞计数;C:横向切片中的细胞计数(同一比例下,对相同大小的红框中的细胞进行计数;细胞数量少则表示细胞长度较长,细胞大小较大。)

Fig.6 Cytological observations of 4CL30 and WT plants Note:A:Transverse and longitudinal paraffin sections of same-node stems of 4CL30 and WT plants;B:Cell counts in longitudinal sections;C:Cell counting in transverse sections (Cells in red boxes of the same size are counted at the same scale; a small number of cells indicates a longer cell length and a larger cell size.)

图7 对照WT和4CL30棉铃大小和纤维长度等表型比较 注:A:从左到右依次是植株棉铃大小、纤维长度和种子大小;B:4CL30和对照WT植株的棉铃重量(同一时期第一果枝上的棉铃);C:4CL30和对照WT植株的纤维长度;D:4CL30和对照WT的衣分;E: 4CL30和对照WT的子指

Fig.7 Phenotypic comparison of boll size and fiber length of WT and 4CL30 plants Note:A:From left to right are plant boll size, fiber length and seed size;B:Cotton boll weight (Cotton bolls on the first fruit branch of the same period) of WT and 4CL30 plants;C:Fiber length of WT and 4CL30 plants;D:Lint percent of WT and 4CL30 plants;E:Seed index of WT and 4CL30 plants