甜玉米基因Sugary1(Su1)序列的变异分析

doi:10.6048/j.issn.1001-4330.2024.07.006

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (7): 1605-1614.DOI: 10.6048/j.issn.1001-4330.2024.07.006

• 作物遗传育种·种质资源·分子遗传学·耕作栽培·生理生化 • 上一篇下一篇

甜玉米基因Sugary1(Su1)序列的变异分析

杨彩霞¹^,²(), 顾炜²^,³, 关媛²^,³, 瞿静涛²^,³, 党冬冬²^,³, 吴鹏昊¹(), 郑洪建²^,³()

1.新疆农业大学农学院,乌鲁木齐 830052
2.上海市农业科学院作物育种栽培研究所/CIMMYT-中国特用玉米研究中心,上海 201403
3.上海特用玉米工程技术研究中心,上海 201403

收稿日期:2023-12-17 出版日期:2024-07-20 发布日期:2024-09-04
通信作者: 吴鹏昊(1984-),男,新疆人,教授,研究方向为玉米遗传育种,(E-mail)craie788@126.com；
郑洪建(1973-),男,山东人,研究员,研究方向为玉米遗传育种,(E-mail)hjzh6188@163.com
作者简介:杨彩霞(1997-),女,重庆人,硕士研究生,研究方向为玉米遗传育种,(E-mail)yangcx4760@163.com
基金资助:
上海市科学技术委员会科技计划项目“鲜食玉米营养、健康功能种质创新与新品种选育”(20392000400);上海市科技兴农项目“糯玉米单倍体诱导系的创制及工程化育种应用”(2022-02-08-00-F01190)

Variation analysis of sweetness gene Sugary1 (Su1) sequence in Sweet corn

YANG Caixia¹^,²(), GU Wei²^,³, GUAN Yuan²^,³, QU Jingtao²^,³, DANG Dongdong²^,³, WU Penghao¹(), ZHENG Hongjian²^,³()

1. College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052, China
2. Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences/CIMMYT-China Special Maize Research Center, Shanghai 201403, China
3. Shanghai Special Maize Engineering Technology Research Center, Shanghai 201403, China

Received:2023-12-17 Published:2024-07-20 Online:2024-09-04
Supported by:
Science and Technology Plan Project of Shanghai Municipal Commission of Science and Technology"Nutritional and Healthy Functional Germplasm Innovation and Breeding of New Varieties of Fresh"(20392000400);Shanghai Science and Technology Agricultural Technology Innovation Project"Creation of haploid-inducing lines in glutinous maize and application to engineering breeding"(2022-02-08-00-F01190)

摘要/Abstract

摘要：

【目的】研究甜玉米基因Sugary1(Su1)序列,分析核苷酸多态性及检验,研究不同来源甜玉米中的Su1的等位变异位点,为甜玉米的品种改良和提高品质提供理论基础。【方法】以B73的Su1基因组序列为模板,对170份甜玉米自交系的Su1基因进行测序,研究玉米Su1基因序列的多态性。【结果】共有97个核苷酸位点发生变异,包含85个SNP位点,12个InDel位点。其中,位于编码区的SNP位点12个,InDel位点0个;位于非编码区的SNP位点85个,InDel位点12个。编码区中SNP位点同义突变有5个,非同义突变7个。自交系材料分为76个单倍型,该基因编码区序列将自交系材料分为47种单倍型。【结论】甜玉米Su1基因经历了明显的人工选择。

关键词: 甜玉米; Sugary1; 等位变异; 中性检验; 单倍型

Abstract:

【Objective】The Sugary1 (Su1) gene of sweet corn was analyzed by nucleotide polymorphism analysis and neutral test, and the allelic variation sites of Su1 in sweet corn from different sources were analyzed, which provided a theoretical basis for variety improvement and quality improvement of sweet corn. 【Methods】In order to illustrate the sequence polymorphism of maize gene Su1, the Su1gene in 170 sweet corn inbred lines was sequenced, using the genomic sequence of B73 as the reference.【Results】Analysis showed that 97 nucleotide sites were mutated, including 85 SNPs sites and 12 InDels locus. Among them, there were 12 SNPs loci and 0 InDel in the coding region, 85 SNPs loci and 12 InDels in the non-coding region. There were 5 synonymous mutations at SNP site and 7 non-synonymous mutations in the coding region. The inbred lines were divided into 76 haplotypes according to the full-length sequence of Su1 gene, and 47 haplotypes were divided into 47 haplotypes according to the coding region of the gene.【Conclusion】The Su1 gene had undergone obvious artificial selection.

Key words: sweet corn; Sugary1; allelic variation; neutral test; haplotype

中图分类号:

S513
S188

杨彩霞, 顾炜, 关媛, 瞿静涛, 党冬冬, 吴鹏昊, 郑洪建. 甜玉米基因Sugary1(Su1)序列的变异分析[J]. 新疆农业科学, 2024, 61(7): 1605-1614.

YANG Caixia, GU Wei, GUAN Yuan, QU Jingtao, DANG Dongdong, WU Penghao, ZHENG Hongjian. Variation analysis of sweetness gene Sugary1 (Su1) sequence in Sweet corn[J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1605-1614.

图/表 8

表1 Su1基因引物序列信息

Tab.1 Primer sequence information of Su1 gene

引物 Primers	序列(5'-3') Sequences(5'-3')	产物长度 Product length(bp)	退火温度 Melting temperature(℃)
1-F	CAGAAACTGATTGCTGGGTG	1 131	60
1-R	GGCAGGGCATTAGCTCTATAC
2-F	AGCTTATAGGAAGAGATGGATAATT	1 445	58
2-R	AGAGGTGTCCCTGTCGTAATC
3-F	CAGATACTGGGTAACAGAAATGC	1 301	58
3-R	GGACTGATAGATGGCTAAATGG
4-F	CCCCTCACTGAATCCCATAAC	1 441	60
4-R	CTTTTCTCTGCTCAACCCACAT
5-F	AGGACTTCCCAGCCCAACT	1 457	58
5-R	CCAGCATACCACTGACTACAAAT
6-F	TCCGAAGATTAAGGAAGAGG	1 467	60
6-R	AACTACCGACAACAGAGCAAC

图1 甜玉米Su1基因引物检测部分材料PCR扩增电泳图注: A:引物1-F/1-R; B:引物2-F/2-R; C:引物3-F/3-R; D:引物4-F/4-R; E:引物5-F/5-R;F:引物6-F/6-R

Fig.1 PCR amplification electrophoresis map of Su1 gene primer detection partial materials in sweet corn Note: A : primer 1-F/1-R; B : primer 2-F/2-R; C :primer 3-F/3-R; D : primer 4-F/4-R; E : Primer 5-F/5-R; F : Primer 6-F/6-R

表2 甜玉米Su1基因编码区多态性

Tab.2 Polymorphism of Su1 gene coding region in sweet corn

位点 Site	多态类型 Polymorphic type	频率 Frequence	位置 Location	密码子变化 Coding change	氨基酸变化 Amino Acid Mutation
InDel_261-269	-/GTCACGA	0.48	intron3
SNP_285	G/A	0.49	intron3
InDel_290	-/T	0.20	intron3
SNP_328	A/G	0.71	intron
InDel_389-419	TCTCGTTTGCAGGTTCAAATGGAGTGAAGTT /-	0.48	intron3
SNP_734	A/T	0.22	intron4
SNP_746	G/A	0.22	intron4
SNP_770	G/T	0.49	intron4
SNP_863	T/C	0.22	intron4
SNP_901	A/G	0.71	intron4
InDel_930	T/-	0.21	intron4
SNP_1106	G/C	0.55	exon5	GTG/GTC	n(V/V)
SNP_1364	A/G	0.18	intron5
SNP_1389	A/G	0.19	intron5
SNP_1403	T/C	0.20	intron5
SNP_1572	A/G	0.59	intron6
SNP_1587	C/T	0.19	intron6
SNP_1836	C/T	0.19	intron7
SNP_1861	A/G	0.18	intron7
SNP_2054	G/A	0.56	intron8
SNP_2067	T/G	0.56	intron8
SNP_2085	T/C	0.36	intron8
SNP_2137	T/C	0.36	intron8
SNP_2498	A/G	0.58	exon10	AAT/AGT	N/S
SNP_2524	A/T	0.50	intron10
InDel_2542	T/-	0.51	intron10
SNP_2571	A/G	0.74	intron10
SNP_2586	G/A	0.24	intron10
SNP_2650	C/T	0.49	intron10
SNP_2713	C/T	0.50	intron10
InDel_2777	-/AAG	0.51	intron10
SNP_2880	C/T	0.74	intron10
SNP_2881	A/G	0.51	intron10
InDel_2910	-/C	0.24	intron10
SNP_3076	T/A	0.24	intron10
SNP_3107	A/G	0.51	intron10
SNP_3432	T/C	0.20	intron10
SNP_3455	A/C	0.74	intron10
InDel_3629-3635	CTGCCA/-	0.18	intron10
SNP_3636	A/G	0.04	intron10
SNP_3664	T/C	0.44	intron10
SNP_3725	C/A	0.45	intron10
SNP_3808	T/C	0.19	intron10
SNP_3856	C/T	0.51	intron10
SNP_4070	A/G	0.52	intron10
SNP_4073	A/G	0.54	intron10
	A/T	0.22	intron10
SNP_4153	T/A	0.76	intron10
SNP_4316	T/C	0.76	intron11
SNP_4322	G/A	0.52	intron11
SNP_4329	G/C	0.22	intron11
SNP_4386	T/A	0.22	intron11
SNP_4479	C/T	0.22	intron11
SNP_4685	C/A	0.21	intron11
SNP_4820	A/C	0.48	intron11
SNP_5037	T/C	0.28	intron11
SNP_5071	G/A	0.50	intron11
SNP_5185	C/G	0.74	intron12
SNP_5292	A/C	0.25	intron12
SNP_5369	G/A	0.24	intron12
SNP_5406	G/T	0.75	intron12
SNP_5503	G/T	0.50	intron12
SNP_5522	G/A	0.49	intron12
SNP_5758	T/C	0.16	intron13
SNP_5845	C/A	0.49	intron13
SNP_6094	T/C	0.75	intron14
SNP_6169	T/C	0.72	intron14
SNP_6283	A/C	0.71	intron14
SNP_6123	G/A	0.25	intron14
SNP_6342	C/A	0.72	intron14
SNP_6391	C/A	0.24	intron14
InDel_6449	T/-	0.24	intron14
SNP_6594	C/A	0.47	intron15
SNP_6597	G/A	0.24	intron15
SNP_6623	A/G	0.47	intron15
InDel_6628	C/-	0.47	intron15
SNP_6679	T/C	0.24	exon16	AAT/AAC	n(N/N)
SNP_6686	C/T	0.47	exon16	CGT/TGT	R/C
SNP_6721	G/T	0.47	exon16	TTG/TTT	L/F
SNP_6746	A/G	0.02	exon16	AAA/GAA	K/E
SNP_6773	C/T	0.47	intron16
SNP_6807	C/A	0.24	intron16
SNP_6870	G/T	0.71	intron16
SNP_6887	A/G	0.71	exon17	AAA/GAA	K/E
SNP_6956	C/T	0.46	exon17	CCC/CCT	n(P/P)
SNP_7053	G/A	0.46	intron17
InDel_7083	G/-	0.23	intron17
InDel_7084	A/-	0.23	intron17
SNP_7086	C/T	0.46	intron17
SNP_7087	G/A	0.23	intron17
SNP_7192	T/C	0.69	exon18	GTT/GTC	n(V/V)
SNP_7218	G/A	0.46	exon18	CGA/CAA	R/Q
SNP_7279	G/A	0.17	exon18	CTG/CTA	n(L/L)
SNP_7379	G/C	0.46	exon18	GAT/CAT	D/H
SNP_7395	A/G	0.69	3'UTR
SNP_7397	A/T	0.46	3'UTR
SNP_7414	A/G	0.69	3'UTR

表2 甜玉米Su1基因编码区多态性

Tab.2 Polymorphism of Su1 gene coding region in sweet corn

位点 Site	多态类型 Polymorphic type	频率 Frequence	位置 Location	密码子变化 Coding change	氨基酸变化 Amino Acid Mutation
InDel_261-269	-/GTCACGA	0.48	intron3
SNP_285	G/A	0.49	intron3
InDel_290	-/T	0.20	intron3
SNP_328	A/G	0.71	intron
InDel_389-419	TCTCGTTTGCAGGTTCAAATGGAGTGAAGTT /-	0.48	intron3
SNP_734	A/T	0.22	intron4
SNP_746	G/A	0.22	intron4
SNP_770	G/T	0.49	intron4
SNP_863	T/C	0.22	intron4
SNP_901	A/G	0.71	intron4
InDel_930	T/-	0.21	intron4
SNP_1106	G/C	0.55	exon5	GTG/GTC	n(V/V)
SNP_1364	A/G	0.18	intron5
SNP_1389	A/G	0.19	intron5
SNP_1403	T/C	0.20	intron5
SNP_1572	A/G	0.59	intron6
SNP_1587	C/T	0.19	intron6
SNP_1836	C/T	0.19	intron7
SNP_1861	A/G	0.18	intron7
SNP_2054	G/A	0.56	intron8
SNP_2067	T/G	0.56	intron8
SNP_2085	T/C	0.36	intron8
SNP_2137	T/C	0.36	intron8
SNP_2498	A/G	0.58	exon10	AAT/AGT	N/S
SNP_2524	A/T	0.50	intron10
InDel_2542	T/-	0.51	intron10
SNP_2571	A/G	0.74	intron10
SNP_2586	G/A	0.24	intron10
SNP_2650	C/T	0.49	intron10
SNP_2713	C/T	0.50	intron10
InDel_2777	-/AAG	0.51	intron10
SNP_2880	C/T	0.74	intron10
SNP_2881	A/G	0.51	intron10
InDel_2910	-/C	0.24	intron10
SNP_3076	T/A	0.24	intron10
SNP_3107	A/G	0.51	intron10
SNP_3432	T/C	0.20	intron10
SNP_3455	A/C	0.74	intron10
InDel_3629-3635	CTGCCA/-	0.18	intron10
SNP_3636	A/G	0.04	intron10
SNP_3664	T/C	0.44	intron10
SNP_3725	C/A	0.45	intron10
SNP_3808	T/C	0.19	intron10
SNP_3856	C/T	0.51	intron10
SNP_4070	A/G	0.52	intron10
SNP_4073	A/G	0.54	intron10
	A/T	0.22	intron10
SNP_4153	T/A	0.76	intron10
SNP_4316	T/C	0.76	intron11
SNP_4322	G/A	0.52	intron11
SNP_4329	G/C	0.22	intron11
SNP_4386	T/A	0.22	intron11
SNP_4479	C/T	0.22	intron11
SNP_4685	C/A	0.21	intron11
SNP_4820	A/C	0.48	intron11
SNP_5037	T/C	0.28	intron11
SNP_5071	G/A	0.50	intron11
SNP_5185	C/G	0.74	intron12
SNP_5292	A/C	0.25	intron12
SNP_5369	G/A	0.24	intron12
SNP_5406	G/T	0.75	intron12
SNP_5503	G/T	0.50	intron12
SNP_5522	G/A	0.49	intron12
SNP_5758	T/C	0.16	intron13
SNP_5845	C/A	0.49	intron13
SNP_6094	T/C	0.75	intron14
SNP_6169	T/C	0.72	intron14
SNP_6283	A/C	0.71	intron14
SNP_6123	G/A	0.25	intron14
SNP_6342	C/A	0.72	intron14
SNP_6391	C/A	0.24	intron14
InDel_6449	T/-	0.24	intron14
SNP_6594	C/A	0.47	intron15
SNP_6597	G/A	0.24	intron15
SNP_6623	A/G	0.47	intron15
InDel_6628	C/-	0.47	intron15
SNP_6679	T/C	0.24	exon16	AAT/AAC	n(N/N)
SNP_6686	C/T	0.47	exon16	CGT/TGT	R/C
SNP_6721	G/T	0.47	exon16	TTG/TTT	L/F
SNP_6746	A/G	0.02	exon16	AAA/GAA	K/E
SNP_6773	C/T	0.47	intron16
SNP_6807	C/A	0.24	intron16
SNP_6870	G/T	0.71	intron16
SNP_6887	A/G	0.71	exon17	AAA/GAA	K/E
SNP_6956	C/T	0.46	exon17	CCC/CCT	n(P/P)
SNP_7053	G/A	0.46	intron17
InDel_7083	G/-	0.23	intron17
InDel_7084	A/-	0.23	intron17
SNP_7086	C/T	0.46	intron17
SNP_7087	G/A	0.23	intron17
SNP_7192	T/C	0.69	exon18	GTT/GTC	n(V/V)
SNP_7218	G/A	0.46	exon18	CGA/CAA	R/Q
SNP_7279	G/A	0.17	exon18	CTG/CTA	n(L/L)
SNP_7379	G/C	0.46	exon18	GAT/CAT	D/H
SNP_7395	A/G	0.69	3'UTR
SNP_7397	A/T	0.46	3'UTR
SNP_7414	A/G	0.69	3'UTR

表3 甜玉米Su1基因序列变异参数

Tab.3 Parameters for the sequence of sweet corn Su1 gene

参数 Parameter	序列长度 Product length (bp)	SNPs+InDel 数目 Number of SNP+Indel	SNP+Indel 变异频率 Frequency of SNP+Indel (bp)	SNP 数目 Number of SNP	SNP 频率 Frequency of SNP (bp)	InDels 数目 Number of Indel	Indel 频率 Frequency of Indel (bp)
全长	7 438	161	0.022	86	0.012	75	0.010
intron3	331	41	0.124	2	0.006	39	0.118
exon4	154	0	0	0	0	0	0
intron4	456	7	0.015	6	0.013	1	0.002
exon5	77	1	0.013	1	0.013	0	0
intron5	247	3	0.012	3	0.012	0	0
exon6	164	0	0	0	0	0	0
intron6	91	2	0.022	2	0.022	0	0
exon7	107	0	0	0	0	0	0
intron7	118	2	0.017	2	0.017	0	0
exon8	85	0	0	0	0	0	0
intron8	270	4	0.015	4	0.015	0	0
exon9	72	0	0	0	0	0	0
intron9	73	0	0	0	0	0	0
exon10	127	1	0.008	1	0.008	0	0
intron10	1716	32	0.019	19	0.011	13	0.008
exon11	87	0	0	0	0	0	0
intron11	776	26	0.034	9	0.012	17	0.022
exon12	94	0	0	0	0	0	0
intron12	437	6	0.014	6	0.014	0	0
exon13	159	1	0.006	1	0.006	0	0
intron13	223	1	0.004	1	0.004	0	0
exon14	84	0	0	0	0	0	0
intron14	421	7	0.017	6	0.014	1	0.002
exon15	84	0	0	0	0	0	0
intron15	93	4	0.043	3	0.032	1	0.011
exon16	79	4	0.051	4	0.051	0	0
intron16	130	3	0.023	3	0.023	0	0
exon17	118	2	0.017	2	0.017	0	0
intron17	125	6	0.048	4	0.032	2	0.016
exon18	258	4	0.016	4	0.016	0	0

表4 中性检验相关参数

Tab.4 Neutral test related parameters

	π	θ	Tajima’s D	Fu and Li’s D^*	Fu and Li’s F^*
全长	0.004 81	0.002 7	4.140 3 $4 * * *$	2.408 2 $4 * *$	3.829 $6 * *$
intron3	0.003 15	0.001 2	2.275 31^*	0.646 81	1.367 3
exon4	0	0	0	0	0
intron4	0.004 31	0.002 31	1.796 59^*	0.569 9	0.774 04
exon5	0.006 47	0.002 27	0	0	0
intron5	0.003 77	0.002 12	1.273 95	0.784 19	1.112 72
exon6	0	0	0	0	0
intron6	0.010 48	0.003 85	2.414 01	0.646 81	1.418 9
exon7	0	0	0	0	0
intron7	0.005 19	0.002 97	1.047 98	0.646 81	0.910 65
exon8	0	0	0	0	0
intron8	0.007 12	0.002 59	3.178 3 $5 * *$	0.896 55	1.952 19^*
exon9	0	0	0	0	0
intron9	0	0	0	0	0
exon10	0.003 96	0.001 38	1.945 52	0.462 13	1.078 16
intron10	0.004 78	0.002 05	3.603 8 $9 * * *$	1.748 2 $9 * *$	2.970 4 $0 * *$
exon11	0	0	0	0	0
intron11	0.004 81	0.002 07	3.058 0 $3 * *$	1.283 1 $7 * *$	2.284 9 $9 * *$
exon12	0	0	0	0	0
intron12	0.005 75	0.002 4	2.893 4 $7 * *$	1.077 5	2.017 07^*
exon13	0	0	0	0	0
intron13	0.002 25	0.000 78	1.945 52	0.462 13	1.078 16
exon14	0	0	0	0	0
intron14	0.005 6	0.002 5	2.572 35^*	1.077 05	1.886 68
exon15	0	0	0	0	0
intron15	0.014 81	0.005 7	2.617 73^*	0.784 19	1.625 72
exon16	0.017 9	0.008 86	1.858 31	0.896 55	1.436 61
intron16	0.009 91	0.004 04	2.387 60^*	0.784 19	1.537 87
exon17	0.007 81	0.002 97	2.285 467^*	0.646 81	1.370 78
intron17	0.013 35	0.005 69	2.450 52^*	0.896 55	1.667 92

表4 中性检验相关参数

Tab.4 Neutral test related parameters

	π	θ	Tajima’s D	Fu and Li’s D^*	Fu and Li’s F^*
全长	0.004 81	0.002 7	4.140 3 $4 * * *$	2.408 2 $4 * *$	3.829 $6 * *$
intron3	0.003 15	0.001 2	2.275 31^*	0.646 81	1.367 3
exon4	0	0	0	0	0
intron4	0.004 31	0.002 31	1.796 59^*	0.569 9	0.774 04
exon5	0.006 47	0.002 27	0	0	0
intron5	0.003 77	0.002 12	1.273 95	0.784 19	1.112 72
exon6	0	0	0	0	0
intron6	0.010 48	0.003 85	2.414 01	0.646 81	1.418 9
exon7	0	0	0	0	0
intron7	0.005 19	0.002 97	1.047 98	0.646 81	0.910 65
exon8	0	0	0	0	0
intron8	0.007 12	0.002 59	3.178 3 $5 * *$	0.896 55	1.952 19^*
exon9	0	0	0	0	0
intron9	0	0	0	0	0
exon10	0.003 96	0.001 38	1.945 52	0.462 13	1.078 16
intron10	0.004 78	0.002 05	3.603 8 $9 * * *$	1.748 2 $9 * *$	2.970 4 $0 * *$
exon11	0	0	0	0	0
intron11	0.004 81	0.002 07	3.058 0 $3 * *$	1.283 1 $7 * *$	2.284 9 $9 * *$
exon12	0	0	0	0	0
intron12	0.005 75	0.002 4	2.893 4 $7 * *$	1.077 5	2.017 07^*
exon13	0	0	0	0	0
intron13	0.002 25	0.000 78	1.945 52	0.462 13	1.078 16
exon14	0	0	0	0	0
intron14	0.005 6	0.002 5	2.572 35^*	1.077 05	1.886 68
exon15	0	0	0	0	0
intron15	0.014 81	0.005 7	2.617 73^*	0.784 19	1.625 72
exon16	0.017 9	0.008 86	1.858 31	0.896 55	1.436 61
intron16	0.009 91	0.004 04	2.387 60^*	0.784 19	1.537 87
exon17	0.007 81	0.002 97	2.285 467^*	0.646 81	1.370 78
intron17	0.013 35	0.005 69	2.450 52^*	0.896 55	1.667 92

图2 甜玉米自交系中Su1基因全长单倍型分布

Fig.2 Distribution of major haplotype of sweet corn Su1 gene total sequences among tested inbred lines

图3 甜玉米自交系中Su1基因编码区单倍型分布

Fig.3 Distribution of major haplotype of sweet corn Su1 gene coding sequences among tested inbred lines

图4 不同CDS单倍型编码的Su1蛋白序列比对

Fig.4 Sequence alignment of the Su1 proteins encoded by different CDS haplotypes

参考文献 23

[1]	陈骁熠, 瞿宏杰. 甜玉米的品质改良技术[J]. 农村经济与科技, 2001, 12(8): 15.
	CHEN Xiaoyi, QU Hongjie. Quality improvement technology of sweet corn[J]. Rural Economy and Science-Technology, 2001, 12(8): 15.
[2]	曹忠良, 张俊江. 甜玉米的类型及关键性技术[J]. 民营科技, 2013, (5): 212.
	CAO Zhongliang, ZHANG Junjiang. Types of sweet corn and key technologies[J]. NonState Running Science Technology Enterprises, 2013, (5): 212.
[3]	蒙云飞, 冯云敢, 贺囡囡, 等. 甜玉米的起源和分布及遗传基因研究进展[J]. 现代农业科技, 2017, (5): 4-5.
	MENG Yunfei, FENG Yungan, HE Nannan, et al. Research progress on origin distribution and genetic genes of sweet corn[J]. Modern Agricultural Science and Technology, 2017, (5): 4-5.
[4]	邵振中. 甜玉米的研究现状与发展[J]. 襄樊职业技术学院学报, 2004, 3(4): 23-25.
	SHAO Zhenzhong. The current situation of research and development of sweet corn[J]. Journal of Xiangfan Vocational and Technical College, 2004, 3(4): 23-25.
[5]	Zhang X, Mogel K J H V, Lor V S, et al. Maizesugary enhancer 1 (se1) is a gene affecting endosperm starch metabolism[J]. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116(41): 20776-20785.
[6]	Ferguson J E, Dickinson D B, Rhodes A M. Analysis of endosperm sugars in a sweet corn inbred (illinois 677a) which contains the sugary enhancer (se) gene and comparison of se with other corn genotypes[J]. Plant Physiology, 1979, 63(3): 416-420.
[7]	Chhabra R, Muthusamy V, Gain N, et al. Allelic variation in sugary1 gene affecting kernel sweetness among diverse-mutant and-wild-type maize inbreds[J]. Molecular Genetics and Genomics: MGG, 2021, 296(5): 1085-1102.
[8]	La Bonte D R, Juvik J A. Characterization ofsugary-1 (su-1) sugary enhancer (se) Kernels in Segregating Sweet Corn Populations[J]. Journal of the American Society for Horticultural Science, 1990, 115(1): 153-157.
[9]	Schultz J A, Juvik J A. Current models for starch synthesis and the sugary enhancer1 (se1) mutation inZea mays[J]. Plant Physiology and Biochemistry: PPB, 2004, 42(6): 457-464.
[10]	Yu J K, Moon Y S. Corn starch: quality and quantity improvement for industrial uses[J]. Plants, 2021, 11(1): 92.
[11]	Pan D, Nelson O E. A debranching enzyme deficiency in endosperms of the sugary-1 mutants of maize[J]. Plant Physiology, 1984, 74(2): 324-328.
[12]	Trimble L, Shuler S, Tracy W F. Characterization of five naturally occurring alleles at the Sugary1 locus for seed composition, seedling emergence, and Isoamylase1 activity[J]. Crop Science, 2016, 56(4): 1927-1939.
[13]	Rahman A, et al. Characterization of SU1 isoamylase, a determinant of storage starch structure in maize[J]. Plant Physiology, 1998, 117(2): 425-435.
[14]	Revilla P, Anibas C M, Tracy W F. Sweet Corn Research around the World 2015-2020[J]. Agronomy, 2021, 11(3): 534.
[15]	Szöke L, Moloi M J, Kovács G E, et al. The application of phytohormones as biostimulants in corn smut infected Hungarian sweet and fodder corn hybrids[J]. Plants, 2021, 10(9): 1822.
[16]	Chourey P S, Nelson O E. The enzymatic deficiency conditioned by the shrunken-1 mutations in maize[J]. Biochemical Genetics, 1976, 14(11/12): 1041-1055.
[17]	Trimble L, Shuler S, Tracy W F. Characterization of Five NaturallyOccurring Alleles at theSugary1Locus for Seed Composition, Seedling Emergence, andIsoamylase1 Activity[J]. Crop Science, 2016, 56(4):1927-1939.
[18]	顾炜. 玉米籽粒突变基因Mn219和Dek39的图位克隆与基因功能分析[D]. 北京: 中国农业大学. 2018.
	GU Wei. The map-based cloning and functional analysis of MN219 and Dek39 in maize[D]. Beijing: China Agricultural University, 2018.
[19]	Rozas J, Ferrer-Mata A, Sánchez-DelBarrio J C, et al. DnaSP 6: DNA sequence polymorphism analysis of large data sets[J]. Molecular Biology and Evolution, 2017, 34(12): 3299-3302.
[20]	Fu Y X, Li W H. Statistical tests of neutrality of mutations[J]. Genetics, 1993, 133(3): 693-709.
[21]	Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism[J]. Genetics, 1989, 123(3): 585-595.
[22]	杨泽峰, 张恩盈, 蒋莹, 等. 玉米耐旱相关基因dbf1的序列变异分析[J]. 玉米科学, 2014, 22(5): 20-28.
	YANG Zefeng, ZHANG Enying, JIANG Ying, et al. Nucleotide diversity of drought tolerance related maize gene dbf1[J]. Journal of Maize Sciences, 2014, 22(5): 20-28.
[23]	Ching A, Caldwell K S, Jung M, et al. SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines[J]. BMC Genetics, 2002, 3: 19.

甜玉米基因Sugary1(Su1)序列的变异分析

Variation analysis of sweetness gene Sugary1 (Su1) sequence in Sweet corn

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