氯化钙采前喷施对西梅采后果实活性氧代谢的调节作用

doi:10.6048/j.issn.1001-4330.2024.08.013

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (8): 1937-1946.DOI: 10.6048/j.issn.1001-4330.2024.08.013

• 园艺特产·农产品加工工程·林业 • 上一篇下一篇

氯化钙采前喷施对西梅采后果实活性氧代谢的调节作用

田全明¹^,²(), 祁华梅³, 殷贝贝⁴, 魏佳⁴, 张政⁴, 王曼⁴, 邢世均², 袁宇尧⁴, 吴斌⁴^,⁵()

1.岭南师范学院,广东湛江 524048
2.新疆农业大学园艺学院,乌鲁木齐 830052
3.新疆大学生命科学与技术学院,乌鲁木齐 830046
4.新疆农业科学院农产品贮藏加工研究所,乌鲁木齐 830091
5.新疆农产品加工与保鲜重点实验室,乌鲁木齐 830091

收稿日期:2024-01-11 出版日期:2024-08-20 发布日期:2024-09-19
通信作者: 吴斌(1973-),男,新疆塔城人,研究员,博士(后),硕士生/博士生导师,研究方向为农产品贮藏与保鲜,(E-mail)42042615@qq.com
作者简介:田全明(1994-),男,河南周口人,博士研究生,研究方向为农产品贮藏与保鲜,(E-mail)1094185168@qq.com
基金资助:
科技创新领军人才项目-高层次领军人才(2022TSYCLJ0040);新疆维吾尔自治区重点研发计划(2022B02026-1)

Regulation of postharvest fruit reactive oxygen metabolism by preharvest spraying of calcium chloride on prunes

TIAN Quanming¹^,²(), QI Huamei³, YAN Beibei⁴, WEI Jia⁴, ZHANG Zheng⁴, WANG Man⁴, XIN Shijun², YUAN Yuyao⁴, WU Bin⁴^,⁵()

1. Lingnan Normal University,Zhanjiang Guangdong 524048, China
2. College of Horticulture,Xinjiang Agricultural University, Urumqi 830052, China,
3. School of Life Science and Technology, Xinjiang University, Urumqi 830046, China
4. Research Institute of Farm Product Storage and Processing,Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
5. Key Laboratory Of Agricultural Products Processing And Preservation, Urumqi 830091, China

Received:2024-01-11 Published:2024-08-20 Online:2024-09-19
Correspondence author: WU Bin(1973-), male, from Tacheng, Xinjiang, researcher,Ph.D.,master/doctoral supervisor,research direction: storage and preservation of agricultural products, (E-mail)42042615@qq.com
Supported by:
Leading Talents in Science and Technology Innovation Project "High-Level Leading Talents(2022TSYCLJ0040);Key R & D Program Project of Xinjiang Uygur Autonomous Region(2022B02026-1)

摘要/Abstract

摘要：

【目的】研究CaCl₂(Calcium chloride)采前喷施对西梅果实采后衰老的抑制作用。【方法】以新疆喀什地区伽师县法兰西西梅为试材,采用2%CaCl₂在果实的膨大期、转色期和采收期喷施,测定活性氧代谢相关酶活性,分析果实衰老与活性代谢之间的关系。【结果】采前CaCl₂处理可延缓西梅采后果实失重率、总酚和类黄酮的下降,抑制西梅采后果实中丙二醛含量的升高、加快清除超氧阴离子、过氧化氢。采前CaCl₂处理显著提高过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、谷胱甘肽还原酶(Glutathione reductase,GR)的活性,从而延缓果实采后失重率的下降。贮藏至第21 d,CaCl₂处理果实的失重率提高了67.05%,POD、SOD、CAT、APX、GR的活性分别提高了30.21%、15.87%、48.41%、57.46%和50.49%。【结论】CaCl₂采前处理减少了活性氧的积累,减轻了西梅果实的氧化损伤,维持细胞膜的完整性,进而延缓了西梅果实的衰老。

关键词: 氯化钙; 西梅; 活性氧代谢; 衰老

Abstract:

【Objective】 To investigate the inhibitory effect of pre-harvest spraying of CaCl₂ (Calcium chloride) on post-harvest senescence of prune fruits.【Methods】 French prunes from Gashi County, Kashgar Prefecture, Xinjiang, as the test material for the study. The relationship between fruit senescence and active metabolism was analyzed by measuring the activities of enzymes related to active oxygen metabolism using 2% CaCl₂ sprayed at the expansion, colour change and harvesting stages of the fruit. 【Results】 The results showed that the pre-harvest CaCl₂ treatment delayed the decrease of post-harvest fruit weight loss, total phenols and flavonoids, and inhibited the increase of malondialdehyde and accelerated the scavenging of superoxide anion and hydrogen peroxide in the post-harvest fruits of prunes. Pre-harvest CaCl₂ treatment significantly increased the activity of Peroxidase (POD), Superoxide Dismutase (SOD), catalase (CAT), Ascorbate Peroxidase (APX) and Glutathione Reductase (GR), thereby delaying the decline in post-harvest fruit weight loss. After 21 days storage,the weight loss rate of fruits treated with CaCl₂ increased by 67.05%.The acitivies of POD、SOD、CAT、APX、GR increased by 30.21%、15.87%、57.46% and 50.49% respectively.【Conclusion】 Therefore, CaCl₂ pre-harvest treatment reduces the accumulation of reactive oxygen species, mitigates oxidative damage in prune fruit, maintains the integrity of cell membranes thus delaying the ageing of prune fruit.

Key words: calcium chloride; prune; active oxygen metabolism; senility

中图分类号:

TS202.1

田全明, 祁华梅, 殷贝贝, 魏佳, 张政, 王曼, 邢世均, 袁宇尧, 吴斌. 氯化钙采前喷施对西梅采后果实活性氧代谢的调节作用[J]. 新疆农业科学, 2024, 61(8): 1937-1946.

TIAN Quanming, QI Huamei, YAN Beibei, WEI Jia, ZHANG Zheng, WANG Man, XIN Shijun, YUAN Yuyao, WU Bin. Regulation of postharvest fruit reactive oxygen metabolism by preharvest spraying of calcium chloride on prunes[J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1937-1946.

图/表 7

图1 CaCl2处理下西梅采后果实失重率的变化

Fig.1 Changes of CaCl2 treatment on weight loss rate of postharvest prune fruit

图2 CaCl2处理下西梅采后果实AsA含量的变化

Fig.2 Changes of CaCl2 treatment on AsA content of postharvest prune fruit

图3 CaCl2处理下西梅采后果实总酚、总黄酮含量的变化

Fig.3 Changes of CaCl2 treatment total phenol and total flavonoid content of postharvest prunes

图4 CaCl2处理下西梅采后果实H2O2、 O 2 · -含量的变化

Fig.4 Changes of CaCl2 treatment on H2O2 and O 2 · - content of postharvest prune fruit

图5 CaCl2处理下西梅采后果实MDA含量的变化

Fig.5 Changes of CaCl2 treatment on MDA content of postharvest prune fruit

图6 CaCl2处理下西梅采后果实POD、SOD活性的变化

Fig.6 Changes of CaCl2 treatment on POD and SOD activities in postharvest prunes

图7 CaCl2处理下西梅采后果实CAT、APX、GR活性的变化

Fig.7 Changes of CaCl2 treatment on CAT, APX and GR activities in postharvest prunes

参考文献 41

[1]	王鹏飞, 付鸿博, 张瑜, 等. 采前喷施脱落酸对欧李果实品质的影响[J]. 核农学报, 2021, 35(9): 2083-2093. DOI
	WANG Pengfei, FU Hongbo, ZHANG Yu, et al. Effects of abscisic acid spraying on fruit quality of Cerasus humilis[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2083-2093. DOI
[2]	新疆维吾尔自治区统计局. 新疆统计年鉴[M]. 北京: 中国统计出版社. 2020: 384-385.
	Xinjiang Statistical Yearbook of Statistics Bureau of Xinjiang Uygur Autonomous Region. Xinjiang Statistical Yearbook[M]. Beijing: China Statistics Press, 2020: 384-385.
[3]	Kosmala M, Milala J, Kołodziejczyk K, et al. Dietary fiber and cell wall polysaccharides from plum (Prunus domestica L.) fruit, juice and pomace: Comparison of composition and functional properties for three plum varieties[J]. Food Research International, 2013, 54(2): 1787-1794.
[4]	Huan C, Jiang L, An X J, et al. Potential role of reactive oxygen species and antioxidant genes in the regulation of peach fruit development and ripening[J]. Plant Physiology and Biochemistry, 2016, (104): 294-303.
[5]	Sharma P, Jha A B, Dubey R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions[J]. Journal of Botany, 2012, 2012: 217037.
[6]	Aboryia M S, El-Gioushy S F, Sami R, et al. Synergistic effect of dipping in Aloe vera gel and mixing with chitosan or calcium chloride on the activities of antioxidant enzymes and cold storage potential of peach (Prunus persica L.) fruits[J]. Coatings, 2022, 12(4): 498.
[7]	Wei D, Zhao X H. Calcium maintained higher quality and enhanced resistance against chilling stress by regulating enzymes in reactive oxygen and biofilm metabolism of Chinese winter jujube fruit[J]. Journal of Food Biochemistry, 2020, 44(4): e13161.
[8]	罗华, 冯立娟, 李敏, 等. 四季草莓“赛娃” 叶片发育过程中光合、MDA含量及SOD、POD活性的变化研究[J]. 山东农业大学学报(自然科学版), 2013, 44(1): 51-55.
	LUO Hua, FENG Lijuan, LI Min, et al. Changes of photosynthetic, mda content and the activity of sod, pod during development of fragaria vesca cv.selva leaves[J]. Journal of Shandong Agricultural University (Natural Science Edition), 2013, 44(1): 51-55.
[9]	Ahmad P, Sarwat M, Ahmad Bhat N, et al. Alleviation of cadmium toxicity in Brassica juncea L. (czern. & coss.) by calcium application involves various physiological and biochemical strategies[J]. PLoS One, 2015, 10(1): e0114571.
[10]	Gao Q Y, Xiong T T, Li X P, et al. Calcium and calcium sensors in fruit development and ripening[J]. Scientia Horticulturae, 2019, (253): 412-421.
[11]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007.
	CAO Jiankang, JIANG Weibo, ZHAO Yumei. Guidance on postharvest physiological and biochemical experiments of fruits and vegetables[M]. Beijing: China Light Industry Press, 2007.
[12]	Xu F X, Liu S Y, Liu Y F, et al. Effectiveness of lysozyme coatings and 1-MCP treatments on storage and preservation of kiwifruit[J]. Food Chemistry, 2019, (288): 201-207.
[13]	李玲玲, 袁航, 叶子, 等. 促干剂对热风条件下西梅品质的影响[J]. 食品工业科技, 2023, 44(11): 238-244.
	LI Lingling, YUAN Hang, YE Zi, et al. Effect of desiccant on the quality of Prunus domestica L. under hot air condition[J]. Science and Technology of Food Industry, 2023, 44(11): 238-244.
[14]	Chumroenphat T, Somboonwatthanakul I, Saensouk S, et al. Changes in curcuminoids and chemical components of turmeric (Curcuma longa L.) under freeze-drying and low-temperature drying methods[J]. Food Chemistry, 2021, (339): 128121.
[15]	Carvalho R L, Cabral M F, Germano T A, et al. Chitosan coating with trans-cinnamaldehyde improves structural integrity and antioxidant metabolism of fresh-cut melon[J]. Postharvest Biology and Technology, 2016, (113): 29-39.
[16]	Song L L, Wang J H, Shafi M, et al. Hypobaric treatment effects on chilling injury, mitochondrial dysfunction, and the ascorbate-glutathione (AsA-GSH) cycle in postharvest peach fruit[J]. Journal of Agricultural and Food Chemistry, 2016, 64(22): 4665-4674. DOI PMID
[17]	Parafati L, Vitale A, Restuccia C, et al. The effect of locust bean gum (LBG)-based edible coatings carrying biocontrol yeasts against Penicillium digitatum and Penicillium italicum causal agents of postharvest decay of mandarin fruit[J]. Food Microbiology, 2016, (58): 87-94.
[18]	Zhu S H, Sun L N, Liu M C, et al. Effect of nitric oxide on reactive oxygen species and antioxidant enzymes in kiwifruit during storage[J]. Journal of the Science of Food and Agriculture, 2008, 88(13): 2324-2331.
[19]	Maurer L H, Bersch A M, Santos R O, et al. Postharvest UV-C irradiation stimulates the non-enzymatic and enzymatic antioxidant system of ‘Isabel’ hybrid grapes (Vitis labrusca × Vitis vinifera L.)[J]. Food Research International, 2017, 102: 738-747.
[20]	张瑜瑜, 用成健, 刘佳妮, 等. 氯化钙处理对蓝莓果实采后生理代谢及品质的影响[J]. 食品研究与开发, 2022, 43(3): 87-93.
	ZHANG Yuyu, YONG Chengjian, LIU Jiani, et al. Effects of CaCl₂Treatment on post-harvest physiology and storage quality of blueberry fruits[J]. Food Research and Development, 2022, 43(3): 87-93.
[21]	曾翔云. 维生素C的生理功能与膳食保障[J]. 中国食物与营养, 2005, 11(4): 52-54.
	ZENG Xiangyun. The physiological function and guarantee measures of vitamin C[J]. Food and Nutrition in China, 2005, 11(4): 52-54.
[22]	吴琼. 冷藏温度与振动胁迫对采后海沃德猕猴桃果实贮藏品质的影响机制研究[D]. 上海: 上海海洋大学, 2018.
	WU Qiong. Research on Effects of Cold Storage Conditions and Vibration Stress on Post-Harvest Storage Quality of Hayward Kiwi Fruit[D]. Shanghai: Shanghai Ocean University, 2018.
[23]	杨莉杰, 李喜宏, 张宇峥, 等. 灵武长枣不同温度贮藏特性的研究[J]. 食品研究与开发, 2018, 39(3): 202-205.
	YANG Lijie, LI Xihong, ZHANG Yuzheng, et al. Storage characteristics of Lingwu long jujubes at different temperatures[J]. Food Research and Development, 2018, 39(3): 202-205.
[24]	乜兰春, 孙建设, 李明. 酚类物质与果蔬品质研究进展[J]. 中国食品学报, 2003, 3(4): 93-98.
	NIE Lanchun, SUN Jianshe, LI Ming. Research progress on the relationship of phenolic compounds and quality of fruits and vegetables[J]. Journal of Chinese Institute of Food Science and Technology, 2003, 3(4): 93-98.
[25]	Parr A J, Bolwell G P. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile[J]. Journal of the Science of Food and Agriculture, 2000, 80(7): 985-1012.
[26]	袁芳, 邱诗铭, 李丽. 不同保鲜剂复合处理对鲜切芒果活性氧代谢、细胞膜透性和褐变的影响[J]. 食品科学, 2020, 41(3): 218-223. DOI
	YUAN Fang, QIU Shiming, LI Li. Effect of composite preservatives on active oxygen metabolism, cell membrane permeability and browning of fresh-cut mango[J]. Food Science, 2020, 41(3): 218-223. DOI
[27]	Meng X Y, Chen C, Song T, et al. Effect of nano-silica coating combined with pressurized Ar treatment on postharvest quality and reactive oxygen species metabolism in sweet cherry fruit[J]. Food Chemistry, 2022, (374): 131715.
[28]	Ge Y H, Li X, Li C Y, et al. Effect of sodium nitroprusside on antioxidative enzymes and the phenylpropanoid pathway in blueberry fruit[J]. Food Chemistry, 2019, (295): 607-612.
[29]	Zhang Y J, Huang Q, Li A R, et al. Apple polyphenols delay postharvest senescence and quality deterioration of ‘Jinshayou’ pummelo fruit during storage[J]. Frontiers in Plant Science, 2022, (13): 1117106.
[30]	Kou X H, He Y L, Li Y F, et al. Effect of abscisic acid (ABA) and chitosan/nano-silica/sodium alginate composite film on the color development and quality of postharvest Chinese winter jujube (Zizyphus jujuba Mill. cv. Dongzao)[J]. Food Chemistry, 2019, (270): 385-394.
[31]	王霞伟, 王曼, 邓豪, 等. 雾化熏蒸结合气调包装技术在冬枣采后贮运保鲜中的应用[J]. 现代食品科技, 2021, 37(10): 153-161.
	WANG Xiawei, WANG Man, DENG Hao, et al. Application of atomization fumigation combined with modified atmosphere packaging technology in storage, transportation and preservation of winter jujube[J]. Modern Food Science and Technology, 2021, 37(10): 153-161.
[32]	Ma Y Y, Fu L, Hussain Z, et al. Enhancement of storability and antioxidant systems of sweet cherry fruit by nitric oxide-releasing chitosan nanoparticles (GSNO-CS NPs)[J]. Food Chemistry, 2019, (285): 10-21.
[33]	Wang Y, Bai J H, Long L E. Quality and physiological responses of two late-season sweet cherry cultivars ‘Lapins’ and ‘Skeena’ to modified atmosphere packaging (MAP) during simulated long distance ocean shipping[J]. Postharvest Biology and Technology, 2015, (110): 1-8.
[34]	吴越, 杨军, 王琦, 等. 贮藏时间和温度对蓝莓花色苷微胶囊品质的影响[J]. 农业工程学报, 2017, 33(8): 301-308.
	WU Yue, YANG Jun, WANG Qi, et al. Effects of storage time and temperature on quality of blueberry anthocyanin microcapsules[J]. Transactions of the Chinese Society of Agricultural Engineering, 2017, 33(8): 301-308.
[35]	杨乐, 侯智霞, 杨俊枫, 等. UV-C对蓝莓酚类物质及其相关酶活性的影响[J]. 浙江农业学报, 2015, 27(6): 955-960.
	YANG Le, HOU Zhixia, YANG Junfeng, et al. Effect of UV-C treatment on phenolic compounds and relevant enzymes activities of blueberry[J]. Acta Agriculturae Zhejiangensis, 2015, 27(6): 955-960.
[36]	黄怡, 高春丽, 毕阳, 等. 低温贮藏期间梨果皮酚类物质及抗氧化性变化[J]. 食品与发酵工业, 2019, 45(19): 219-226. DOI
	HUANG Yi, GAO Chunli, BI Yang, et al. Changes in phenolic compounds and antioxidant activity of pear peel during cold storage[J]. Food and Fermentation Industries, 2019, 45(19): 219-226. DOI
[37]	毕阳, 郭玉蓉, 李永才, 等. 冷藏期间三种梨果皮中酚类物质含量及多酚氧化酶活性变化与褐变度的关系[J]. 制冷学报, 2002, 23(4): 52-54.
	BI Yang, GUO Yurong, LI Yongcai, et al. Changes in peel of three cultivars pears polyphenol concentrations and polyphenoloxidase in relation to degree of browning during cold storage[J]. Refrigeration Journal, 2002, 23(4): 52-54.
[38]	Li D, Cheng Y D, Dong Y, et al. Effects of low temperature conditioning on fruit quality and peel browning spot in ‘Huangguan’ pears during cold storage[J]. Postharvest Biology and Technology, 2017, (131): 68-73.
[39]	Tian S P, Qin G Z, Li B Q. Reactive oxygen species involved in regulating fruit senescence and fungal pathogenicity[J]. Plant Molecular Biology, 2013, 82(6): 593-602. DOI PMID
[40]	Shewfelt R L, del Rosario B A. The role of lipid peroxidation in storage disorders of fresh fruits and vegetables[J]. HortScience, 2000, 35(4): 575-579.
[41]	Koushesh Saba M, Moradi S. Sodium nitroprusside (SNP) spray to maintain fruit quality and alleviate postharvest chilling injury of peach fruit[J]. Scientia Horticulturae, 2017, (216): 193-199.

氯化钙采前喷施对西梅采后果实活性氧代谢的调节作用

Regulation of postharvest fruit reactive oxygen metabolism by preharvest spraying of calcium chloride on prunes

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 41

相关文章 10

编辑推荐

Metrics

[1]	李自芹, 李文绮, 陶登峰, 贾文婷, 金新文, 雷用东, 刘成江. 不同预冷方式结合H₂O₂熏蒸处理对西梅贮藏期间品质的影响[J]. 新疆农业科学, 2024, 61(8): 1947-1954.
[2]	田全明, 殷贝贝, 祁华梅, 魏佳, 张政, 邢世均, 王曼, 袁宇尧, 吴斌. 采前喷施CaCl₂对西梅采后果实软化品质的影响[J]. 新疆农业科学, 2024, 61(7): 1717-1726.
[3]	李自芹, 李文绮, 贾文婷, 李宇辉, 刘成江. 氯化钙与1-MCP对西州蜜甜瓜采后贮藏品质的影响[J]. 新疆农业科学, 2023, 60(7): 1698-1704.
[4]	杨世英, 马玉娥, 贾斌鑫, 华震宇, 沈琦, 刘峰娟, 王成. 油菜素内酯对甜瓜农药残留的降解作用分析[J]. 新疆农业科学, 2023, 60(10): 2541-2550.
[5]	阿塔吾拉·铁木尔, 阿布来克·尼牙孜, 田全明, 张健, 徐斌, 吴斌. “吊挂式”窖藏对冬季白菜贮藏品质及活性氧代谢的保鲜作用分析[J]. 新疆农业科学, 2022, 59(5): 1292-1300.
[6]	刘志刚, 任红松, 胡西单·买买提, 阿木提·库尔班, 郭红梅, 王瑞华, 李海峰, 胡国智. 氨基酸硒叶面肥对甜瓜生长发育和光合特性的影响[J]. 新疆农业科学, 2022, 59(4): 874-883.
[7]	刘志刚, 任红松, 买买提·艾合买提, 胡西单·买买提, 努尔孜叶古丽·马合木提, 阿木提·库尔班, 王瑞华, 李海峰. 氨基酸硒叶面肥对吐鲁番秋季露地甜瓜叶片早衰生理特性的影响[J]. 新疆农业科学, 2021, 58(6): 1078-1085.
[8]	马卉, 牛玉萍, 夏军, 陈宗奎, 罗宏海. 滴灌模式和种植密度对棉花叶片衰老特性的影响[J]. 新疆农业科学, 2017, 54(11): 1972-1982.
[9]	努尔曼·阿不拉;沙比尔江·阿巴克;阿卜杜许库尔·牙合甫;故丽米热·卡克什. 5个西梅品种光合特性、叶绿素及产量的对比研究[J]. , 2013, 50(9): 1626-1631.
[10]	努尔曼·阿不拉;沙比尔江·阿巴克;热沙来提·艾孜孜;木合塔尔·扎热. 5个西梅品种光响应和CO2响应及叶片部分特性差异[J]. , 2012, 49(12): 2189-2195.