新疆农业科学 ›› 2024, Vol. 61 ›› Issue (10): 2417-2426.DOI: 10.6048/j.issn.1001-4330.2024.10.009
收稿日期:
2024-03-15
出版日期:
2024-10-20
发布日期:
2024-11-07
通信作者:
罗瑞峰(1975-),男,新疆石河子人,副研究员,研究方向为农产品质量与安全,(E-mail)1785605310@qq.com作者简介:
张金荣(1996-),女,新疆库尔勒人,硕士研究生,研究方向为农产品加工,(E-mail)419707872@qq.com
基金资助:
ZHANG Jinrong(), LU Shiling, LUO Ruifeng(), MA Xiaoning, WANG Guodong
Received:
2024-03-15
Published:
2024-10-20
Online:
2024-11-07
Correspondence author:
LUO Ruifeng(1975-), male, from Shihezi, Xinjiang, associate research fellow, research direction: agricultural product quality and safety, (E-mail)1785605310@qq.comSupported by:
摘要:
【目的】 研究硒肥浓度和施用时期对葡萄果实硒含量和综合品质的影响,为富硒葡萄的生产和硒肥的合理施用提供理论依据。【方法】 采用大田试验,以克瑞森无核(小粒)、克瑞森无核(大粒)、阳光玫瑰为材料,分析叶面喷施纳米硒肥对3种葡萄品质和硒含量的影响。【结果】 3种葡萄果实总硒含量均随施硒量的增加而升高;在一定施硒浓度下,大幼果期喷施硒肥3种葡萄果实总硒含量达到最大值。其中,阳光玫瑰在不同施硒浓度和施用时期吸收富集硒能力优于克瑞森无核(小粒)和克瑞森无核(大粒),在大幼果期阳光玫瑰硒含量最高为0.176 mg/kg。叶面硒肥通过影响3种葡萄的可溶性固形物、总酸、总糖和原花青素含量从而改善果实品质,但对外观指标及白藜芦醇苷含量影响不显著。【结论】 3种葡萄最适喷施时期为大幼果期,最适叶喷浓度为9.15 L/hm2。
张金荣, 卢士玲, 罗瑞峰, 马小宁, 王国栋. 叶面喷施硒肥对3种葡萄果实硒含量及品质的影响[J]. 新疆农业科学, 2024, 61(10): 2417-2426.
ZHANG Jinrong, LU Shiling, LUO Ruifeng, MA Xiaoning, WANG Guodong. Effect of spraying foliar selenium on selenium content and quality of three cultivars of grape fruit[J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2417-2426.
步骤 Step | 时间 Time (min) | 流动相A The mobile phase A (%) | 流动相B The mobile phase B (%) |
---|---|---|---|
1 | 0 | 100 | 0 |
2 | 5 | 97 | 3 |
3 | 10 | 80 | 20 |
4 | 14 | 80 | 20 |
5 | 14.01 | 97 | 3 |
6 | 30 | 97 | 3 |
表1 梯度洗脱程序
Tab.1 Mobile phase gradient
步骤 Step | 时间 Time (min) | 流动相A The mobile phase A (%) | 流动相B The mobile phase B (%) |
---|---|---|---|
1 | 0 | 100 | 0 |
2 | 5 | 97 | 3 |
3 | 10 | 80 | 20 |
4 | 14 | 80 | 20 |
5 | 14.01 | 97 | 3 |
6 | 30 | 97 | 3 |
处理 Treatments | 品种Species | ||
---|---|---|---|
克瑞森无核 (小粒) Creessen Seedless (small grains) | 克瑞森无核 (大粒) Creessen Seedless (large grains) | 阳光玫瑰 Shine Muscat | |
CK | 0.001±0.001e | 0.000±0.000e | 0.001±0.001e |
4.65 (L/hm2) | 0.007±0.002d | 0.004±0.001d | 0.008±0.000d |
9.15 (L/hm2) | 0.017±0.001c | 0.014±0.000c | 0.016±0.001c |
13.80 (L/hm2) | 0.029±0.001b | 0.028±0.002b | 0.032±0.001b |
18.30 (L/hm2) | 0.035±0.001a | 0.031±0.001a | 0.054±0.002a |
小幼果期 Small fruit stage | 0.016±0.001d | 0.018±0.001d | 0.034±0.001c |
大幼果期 Large fruit stage | 0.098±0.002a | 0.087±0.005a | 0.176±0.006a |
转色期 Color change stage | 0.044±0.003c | 0.065±0.003b | 0.081±0.002b |
成熟前期 Early ripening stage | 0.074±0.002b | 0.051±0.003c | 0.037±0.001c |
表2 不同处理下葡萄果实硒含量的变化
Tab.2 Changes of selenium content of grape berry under different treatments
处理 Treatments | 品种Species | ||
---|---|---|---|
克瑞森无核 (小粒) Creessen Seedless (small grains) | 克瑞森无核 (大粒) Creessen Seedless (large grains) | 阳光玫瑰 Shine Muscat | |
CK | 0.001±0.001e | 0.000±0.000e | 0.001±0.001e |
4.65 (L/hm2) | 0.007±0.002d | 0.004±0.001d | 0.008±0.000d |
9.15 (L/hm2) | 0.017±0.001c | 0.014±0.000c | 0.016±0.001c |
13.80 (L/hm2) | 0.029±0.001b | 0.028±0.002b | 0.032±0.001b |
18.30 (L/hm2) | 0.035±0.001a | 0.031±0.001a | 0.054±0.002a |
小幼果期 Small fruit stage | 0.016±0.001d | 0.018±0.001d | 0.034±0.001c |
大幼果期 Large fruit stage | 0.098±0.002a | 0.087±0.005a | 0.176±0.006a |
转色期 Color change stage | 0.044±0.003c | 0.065±0.003b | 0.081±0.002b |
成熟前期 Early ripening stage | 0.074±0.002b | 0.051±0.003c | 0.037±0.001c |
品种 Species | 施硒梯度 Application of selenium gradient (L/hm2) | 单果重 Single fruit weight(g) | 果形指数 Fruit shape index | 硬度 Hardness (cm2/kg) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 3.70±0.46a | 1.38±0.10ab | 0.95±0.17ab |
4.65 | 4.10±0.60a | 1.35±0.10ab | 0.97±0.10ab | |
9.15 | 4.03±0.45a | 1.29±0.12b | 0.86±0.09b | |
13.80 | 3.83±0.35a | 1.41±0.11a | 1.09±0.17a | |
18.30 | 4.10±0.20a | 1.45±0.14a | 0.98±0.19ab | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 6.80±0.56a | 1.22±0.13b | 0.69±0.13c |
4.65 | 5.90±0.56ab | 1.29±0.07b | 0.81±0.09ab | |
9.15 | 6.10±0.56ab | 1.28±0.05b | 0.74±0.14bc | |
13.80 | 6.90±0.66a | 1.30±0.16b | 0.88±0.10a | |
18.30 | 5.83±0.25ab | 1.52±0.15a | 0.69±0.19c | |
阳光玫瑰 Shine Muscat | CK | 6.60±0.72a | 1.18±0.04a | 0.58±0.04b |
4.65 | 6.43±0.76a | 1.12±0.10a | 0.77±0.11a | |
9.15 | 6.87±0.40a | 1.15±0.06a | 0.62±0.04b | |
13.80 | 7.10±0.62a | 1.12±0.08a | 0.83±0.15a | |
18.30 | 6.93±0.12a | 1.09±0.06a | 0.66±0.10b |
表3 叶面喷施不同浓度硒肥葡萄果实外观品质的变化
Tab.3 Changes of appearance quality of grape berry foliar spraying of different concentrations of selenium fertilizer
品种 Species | 施硒梯度 Application of selenium gradient (L/hm2) | 单果重 Single fruit weight(g) | 果形指数 Fruit shape index | 硬度 Hardness (cm2/kg) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 3.70±0.46a | 1.38±0.10ab | 0.95±0.17ab |
4.65 | 4.10±0.60a | 1.35±0.10ab | 0.97±0.10ab | |
9.15 | 4.03±0.45a | 1.29±0.12b | 0.86±0.09b | |
13.80 | 3.83±0.35a | 1.41±0.11a | 1.09±0.17a | |
18.30 | 4.10±0.20a | 1.45±0.14a | 0.98±0.19ab | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 6.80±0.56a | 1.22±0.13b | 0.69±0.13c |
4.65 | 5.90±0.56ab | 1.29±0.07b | 0.81±0.09ab | |
9.15 | 6.10±0.56ab | 1.28±0.05b | 0.74±0.14bc | |
13.80 | 6.90±0.66a | 1.30±0.16b | 0.88±0.10a | |
18.30 | 5.83±0.25ab | 1.52±0.15a | 0.69±0.19c | |
阳光玫瑰 Shine Muscat | CK | 6.60±0.72a | 1.18±0.04a | 0.58±0.04b |
4.65 | 6.43±0.76a | 1.12±0.10a | 0.77±0.11a | |
9.15 | 6.87±0.40a | 1.15±0.06a | 0.62±0.04b | |
13.80 | 7.10±0.62a | 1.12±0.08a | 0.83±0.15a | |
18.30 | 6.93±0.12a | 1.09±0.06a | 0.66±0.10b |
品种 Species | 施硒时期 Selenium application period | 单果重 Single fruit weight(g) | 果形指数 Fruit shape index | 硬度 Hardness (cm2/kg) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 3.70±0.46b | 1.38±0.10b | 0.95±0.17a |
小幼果期 | 4.03±0.65ab | 1.26±0.05c | 0.73±0.16c | |
大幼果期 | 4.27±0.47ab | 1.32±0.10bc | 0.85±0.15b | |
转色期 | 4.63±0.35a | 1.41±0.10a | 1.06±0.11a | |
成熟前期 | 3.67±0.15b | 1.33±0.10bc | 0.99±0.15a | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 6.80±0.56a | 1.22±0.13b | 0.69±0.13b |
小幼果期 | 5.33±0.50c | 1.25±0.10b | 0.78±0.15ab | |
大幼果期 | 6.43±0.51b | 1.40±0.12a | 0.81±0.13ab | |
转色期 | 7.00±0.46a | 1.37±0.09a | 0.87±0.15a | |
成熟前期 | 6.63±0.57ab | 1.39±0.06a | 0.77±0.08ab | |
阳光玫瑰 Shine Muscat | CK | 6.60±0.72a | 1.18±0.04a | 0.58±0.04c |
小幼果期 | 6.73±0.61a | 1.21±0.08a | 0.80±0.10a | |
大幼果期 | 6.60±0.44a | 1.23±0.05a | 0.70±0.09b | |
转色期 | 6.07±0.80a | 1.18±0.06a | 0.66±0.04b | |
成熟前期 | 6.70±0.36a | 1.18±0.08a | 0.70±0.06b |
表4 不同生长时期喷施硒肥葡萄果实外观品质的变化
Tab.4 Changes of appearance quality of grape berry sprayed with selenium fertilizer in different growth periods
品种 Species | 施硒时期 Selenium application period | 单果重 Single fruit weight(g) | 果形指数 Fruit shape index | 硬度 Hardness (cm2/kg) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 3.70±0.46b | 1.38±0.10b | 0.95±0.17a |
小幼果期 | 4.03±0.65ab | 1.26±0.05c | 0.73±0.16c | |
大幼果期 | 4.27±0.47ab | 1.32±0.10bc | 0.85±0.15b | |
转色期 | 4.63±0.35a | 1.41±0.10a | 1.06±0.11a | |
成熟前期 | 3.67±0.15b | 1.33±0.10bc | 0.99±0.15a | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 6.80±0.56a | 1.22±0.13b | 0.69±0.13b |
小幼果期 | 5.33±0.50c | 1.25±0.10b | 0.78±0.15ab | |
大幼果期 | 6.43±0.51b | 1.40±0.12a | 0.81±0.13ab | |
转色期 | 7.00±0.46a | 1.37±0.09a | 0.87±0.15a | |
成熟前期 | 6.63±0.57ab | 1.39±0.06a | 0.77±0.08ab | |
阳光玫瑰 Shine Muscat | CK | 6.60±0.72a | 1.18±0.04a | 0.58±0.04c |
小幼果期 | 6.73±0.61a | 1.21±0.08a | 0.80±0.10a | |
大幼果期 | 6.60±0.44a | 1.23±0.05a | 0.70±0.09b | |
转色期 | 6.07±0.80a | 1.18±0.06a | 0.66±0.04b | |
成熟前期 | 6.70±0.36a | 1.18±0.08a | 0.70±0.06b |
品种 Species | 施硒梯度 Application of selenium gradient (L/hm2) | 可溶性固形物 Soluble solids (%) | 总酸 Total acid (g/kg) | 总糖(转化后还原糖) Total sugars (reducing sugars after conversion) (g/100 g) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 22.30±1.20ab | 6.51±0.61a | 16.90±0.30a |
4.65 | 20.40±0.20b | 6.31±0.79a | 16.30±0.30b | |
9.15 | 25.05±0.45a | 6.01±0.11a | 16.00±0.20b | |
13.80 | 22.85±2.65ab | 6.55±0.88a | 15.05±0.15c | |
18.30 | 23.60±3.20ab | 7.13±1.17a | 17.15±0.45a | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 16.45±0.35b | 5.85±0.31a | 12.55±1.45c |
4.65 | 21.30±1.20a | 4.46±0.44b | 18.00±0.20a | |
9.15 | 15.90±1.50b | 5.34±0.42ab | 12.75±0.05c | |
13.80 | 15.45±0.65b | 6.23±1.11a | 13.05±0.75c | |
18.30 | 16.95±0.55b | 6.09±0.60a | 14.45±0.55b | |
阳光玫瑰 Shine Muscat | CK | 21.45±0.65a | 4.00±0.10c | 17.20±0.40a |
4.65 | 16.85±0.25c | 6.23±0.42a | 13.80±0.10c | |
9.15 | 18.95±1.05b | 5.72±0.30b | 15.85±0.15b | |
13.80 | 18.30±0.30b | 5.69±0.21b | 15.20±0.40b | |
18.30 | 19.30±0.70b | 4.94±0.10c | 15.20±0.40b |
表5 叶面喷施不同浓度硒肥葡萄果实营养品质的变化
Tab.5 Changes of foliar spraying of different concentrations of selenium fertilizer on grape berry taste quality
品种 Species | 施硒梯度 Application of selenium gradient (L/hm2) | 可溶性固形物 Soluble solids (%) | 总酸 Total acid (g/kg) | 总糖(转化后还原糖) Total sugars (reducing sugars after conversion) (g/100 g) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 22.30±1.20ab | 6.51±0.61a | 16.90±0.30a |
4.65 | 20.40±0.20b | 6.31±0.79a | 16.30±0.30b | |
9.15 | 25.05±0.45a | 6.01±0.11a | 16.00±0.20b | |
13.80 | 22.85±2.65ab | 6.55±0.88a | 15.05±0.15c | |
18.30 | 23.60±3.20ab | 7.13±1.17a | 17.15±0.45a | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 16.45±0.35b | 5.85±0.31a | 12.55±1.45c |
4.65 | 21.30±1.20a | 4.46±0.44b | 18.00±0.20a | |
9.15 | 15.90±1.50b | 5.34±0.42ab | 12.75±0.05c | |
13.80 | 15.45±0.65b | 6.23±1.11a | 13.05±0.75c | |
18.30 | 16.95±0.55b | 6.09±0.60a | 14.45±0.55b | |
阳光玫瑰 Shine Muscat | CK | 21.45±0.65a | 4.00±0.10c | 17.20±0.40a |
4.65 | 16.85±0.25c | 6.23±0.42a | 13.80±0.10c | |
9.15 | 18.95±1.05b | 5.72±0.30b | 15.85±0.15b | |
13.80 | 18.30±0.30b | 5.69±0.21b | 15.20±0.40b | |
18.30 | 19.30±0.70b | 4.94±0.10c | 15.20±0.40b |
品种 Species | 施硒时期 Selenium application period | 可溶性固形 Soluble solids (%) | 总酸 Total acid (g/kg) | 总糖 (转化后还原糖) Total sugars (reducing sugars after conversion) (g/100 g) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 22.30±1.20ab | 6.51±0.61a | 16.90±0.30a |
小幼果期 | 21.80±1.60b | 4.78±0.58c | 14.70±0.10b | |
大幼果期 | 20.15±1.35bc | 4.40±0.48c | 15.55±0.75ab | |
转色期 | 23.55±1.05a | 5.79±0.35b | 17.00±1.20a | |
成熟前期 | 18.50±0.10c | 7.16±0.68a | 15.20±0.20b | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 16.45±0.35bc | 5.85±0.31b | 12.55±1.45c |
小幼果期 | 20.00±0.50a | 6.56±0.75ab | 13.00±0.40bc | |
大幼果期 | 15.35±0.75c | 8.17±2.25a | 13.60±0.60bc | |
转色期 | 18.10±1.30b | 5.24±1.18b | 12.70±1.10c | |
成熟前期 | 17.50±0.30b | 5.63±0.71b | 16.10±0.50a | |
阳光玫瑰 Shine Muscat | CK | 21.45±0.65b | 4.00±0.10b | 17.20±0.40c |
小幼果期 | 24.45±2.05a | 3.60±0.02bc | 20.05±0.55a | |
大幼果期 | 21.55±0.95b | 3.42±0.26bc | 18.90±0.50b | |
转色期 | 20.05±0.95bc | 3.97±0.13b | 15.00±0.20d | |
成熟前期 | 18.85±0.05c | 5.68±0.18a | 15.50±0.50d |
表6 不同生长时期喷施硒肥葡萄果实营养品质的变化
Tab.6 Changes of taste quality of grape berry sprayed with selenium fertilizer in different growth periods
品种 Species | 施硒时期 Selenium application period | 可溶性固形 Soluble solids (%) | 总酸 Total acid (g/kg) | 总糖 (转化后还原糖) Total sugars (reducing sugars after conversion) (g/100 g) |
---|---|---|---|---|
克瑞森无核(小粒) Creessen Seedless (small grains) | CK | 22.30±1.20ab | 6.51±0.61a | 16.90±0.30a |
小幼果期 | 21.80±1.60b | 4.78±0.58c | 14.70±0.10b | |
大幼果期 | 20.15±1.35bc | 4.40±0.48c | 15.55±0.75ab | |
转色期 | 23.55±1.05a | 5.79±0.35b | 17.00±1.20a | |
成熟前期 | 18.50±0.10c | 7.16±0.68a | 15.20±0.20b | |
克瑞森无核(大粒) Creessen Seedless (large grains) | CK | 16.45±0.35bc | 5.85±0.31b | 12.55±1.45c |
小幼果期 | 20.00±0.50a | 6.56±0.75ab | 13.00±0.40bc | |
大幼果期 | 15.35±0.75c | 8.17±2.25a | 13.60±0.60bc | |
转色期 | 18.10±1.30b | 5.24±1.18b | 12.70±1.10c | |
成熟前期 | 17.50±0.30b | 5.63±0.71b | 16.10±0.50a | |
阳光玫瑰 Shine Muscat | CK | 21.45±0.65b | 4.00±0.10b | 17.20±0.40c |
小幼果期 | 24.45±2.05a | 3.60±0.02bc | 20.05±0.55a | |
大幼果期 | 21.55±0.95b | 3.42±0.26bc | 18.90±0.50b | |
转色期 | 20.05±0.95bc | 3.97±0.13b | 15.00±0.20d | |
成熟前期 | 18.85±0.05c | 5.68±0.18a | 15.50±0.50d |
图1 叶面喷施不同浓度硒肥葡萄果实白藜芦醇苷含量的变化
Fig.1 Changes of content of resveratrol glycosides in grape berry by foliar spraying with different concentrations of selenium fertilizer
图2 不同生长时期喷施硒肥葡萄果实白藜芦醇苷含量的变化
Fig.2 Changes of resveratrol glycosides content of grape berry sprayed with selenium fertilizer in different growth periods
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