Abstract: [Objective] A high performance liquid chromatography (HPLC) method was developed to determine the concentration of four endogenous hormones [indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), zeatin (ZT)] in young fruit of Korla fragrant pear.[Method]After screening and optimization of conditions, 80; methanol was chosen as extraction solvent.Polyvinylpolypyrrolidone (PVPP) was used to absorb impurities.The samples were extracted with petroleum ether and ethyl acetate.The extracts were purified with a reverse pHase Sep-Pak C18 column (250 mm × 4.6 mm, 5 μm particle size).The column temperature was 40 ℃.The flow rate was 1 mL/min.The injection volume was 10 μL.The mobile phase was 45; methanol (A) and 55; acetic acid (B, pH=3.5).The detection wavelength was 254 nm.[Result]ZT, GA3, IAA, abscisic acid analysis method of high performance liquid chromatography was established.The HPLC recovery of known concentrations of IAA, ABA, GA3, and ZT ranged between 80.4; and 91.2;.The relative standard deviations (RSDs) ranged between 1.54; and 8.1;.The experimental results were stable and reliable.[Conclusion]The HPLC method developed in this study is simple, rapid, reproducible, and accurate.Therefore, it is suitable for rapid and accurate quantification of ZT, GA3, IAA and ABA in pear fruit.

摘要： [目的]建立高效液相色谱法(HPLC),用于同时分离和测定库尔勒香梨幼果中的玉米素(ZT)、赤霉素(GA3)、生长素(IAA)和脱落酸(ABA)4种植物内源激素.[方法]经过条件筛选和优化,选用80;甲醇作为样品提取试剂.经过石油醚脱色,PVPP吸附杂质和乙酸乙酯萃取,再经Sep-Pak C18小柱纯化.采用SGE ProteCol-P C18(250 mm×4.6 mm,5 μm)色谱柱,柱温40℃,流速为1 mL/min,进样量10 μL.流动相A为甲醇,流动相B为水(pH=3.5),流动相比例为:45; (A) 与55;(B).检测器波长为254 nm.[结果]建立了玉米素、赤霉素、生长素、脱落酸的高效液相色谱分析方法.运用该方法测得加标回收率达80.4;~91.2;, 相对偏差在1.54;~8.1;,分离效果理想,实验效果稳定可靠.[结论]该方法前处理方法简便,分析速度快,具有较高的重现性和精确性.适用于香梨幼果中内源激素玉米素、赤霉素、生长素和脱落酸的测定分析.