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GUO Gang;LU Yue-min;ZHANG Xiao-hong;FU Xi-ke;Ma Dun;ZHOU Xiao-yun
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郭刚;卢跃敏;张晓宏;付习科;马盾;周小云
摘要: [目的]研究苦马豆的组织培养与快速繁殖的方法.[方法]以苦马豆上胚轴为外植体,MS为基本培养基,通过不同的激素种类和浓度配比,建立苦马豆组培快繁体系.[结果]组织培养与快速繁殖的培养基为:诱导愈伤培养基为MS+6-BA2.0mg/L+2,4-D 0.25 mg/L;继代培养基为MS+6-BA 2.0 mg/L+NA.A0.5 mg/L;诱芽培养基为MS+NAA 0.05 mg/L+ZT0.05 mg/L+AC 0.1;;生根培养基为1/2 MS+ IBA1.5 mg/L+蔗糖(Suc)3;.该组培苗适宜移栽于珍珠岩+蛭石(1∶1)基质中,平均成活率80;.[结论]筛选获得适宜苦马豆上胚轴为外植体的组培快繁的培养条件,为苦马豆快繁体系的建立探索出了一条新的有效的途径.
GUO Gang;LU Yue-min;ZHANG Xiao-hong;FU Xi-ke;Ma Dun;ZHOU Xiao-yun. Tissue Culture and Rapid Propagation of Swainsona salsula[J]. .
郭刚;卢跃敏;张晓宏;付习科;马盾;周小云. 苦马豆的组织培养与快速繁殖[J]. .
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