Abstract: [Objective] The present study aims to understand the salt tolerance of two kinds of Chinese wolfberry seedling and provide the theoretical basis for the large-scale popularization and the utilization of saline-alkali land and sandy wasteland of Chinese wolfberry.[Method]Using the seedlings of Lycium ruthenicum and Lycium barbarum as test materials, watered with NaCl and Na2CO3 solution to study the effects of salt stress on seedlings physiological characteristics of Lycium bararum L.[Result]The results showed that with the increase of NaCl and Na2CO3 concentration, the cell membrane permeability, malondialdehyde, proline and soluble sugar content increased significantly in different time points, Soluble protein content and SOD, POD, CAT enzyme activity showed an upward trend in the early stage of treatment (10 d-30 d), the later treatment showed a trend of increasing and then decreasing t (40 d-50 d).With the increase of NaCl and Na2CO3 processing days, under the same salt concentration, the cell membrane permeability, malondialdehyde, proline and soluble sugar content increased significantly, the soluble protein content and SOD, POD enzyme activity showed a trend of increasing first and then decreasing, and the activity of CAT enzyme showed a downward trend.The effects of Na2CO3 stress on seedling were more significant than that of NaCl stress, the change trend of Lycium chincnse Ningqi 7 was higher than Lycium chincnse Heiqi 1, and with the increase of treatment concentration and treatment days, the degree of injury was increased significantly.[Conclusion]Under the stresses of NaCl and Na2CO3 in the two varieties Chinese wolfberry, malondialdehyde, proline, soluble sugar and the activity of antioxidant enzyme SOD are more sensitive and could be used as the main index to evaluate the salt tolerance of Lycium barbarum.In addition, the seedlings of Lycium chincnse Heiqi 1 and Lycium chincnse Ningqi 7 reached the threshold value at 1.2 mol/L and 1.0 mol/L and the limit value of salt tolerance at 1.5 mol/L under the stress of NaCl, while reached the threshold value at 0.2 mol/L and 0.1 mol/L and the limit value of salt tolerance at 0.5 and 0.4 mol/L under the stress of Na2CO3, which implicated that Lycium chincnse Heiqi 1 had the stronger salt tolerance than Lycium chincnse Ningqi 7, and the effect of Na2CO3 on the seedlings of Chinese wolfberry was significantly greater than that of NaCl.

摘要： [目的]研究不同盐浓度胁迫下两种枸杞幼苗的耐盐性,为枸杞的大面积推广和盐碱地、沙荒地利用提供理论依据.[方法]以黑果枸杞和宁夏枸杞的幼苗为试材,采用不同浓度NaCl和Na2CO3溶液进行胁迫处理后,对其叶片相关生理指标进行测定.[结果]随着NaCl和 Na2CO3处理浓度的增加,质膜透性、丙二醛(MDA)、脯氨酸和可溶性糖含量在不同处理时期均呈现上升趋势,可溶性蛋白含量和SOD、POD、CAT酶活性,在处理前期呈现上升趋势,处理后期呈现先升高后下降的趋势.随着NaCl和 Na2CO3处理天数的增加,同一处理浓度下的质膜透性、丙二醛(MDA)、脯氨酸和可溶性糖含量呈现上升的趋势,可溶性蛋白含量和SOD、POD酶活性,呈现先升高后下降的趋势,CAT酶活性呈现持续下降的趋势.Na2CO3处理后的变化趋势大于NaCl,宁杞7号的变化趋势大于黑杞1号.[结论]黑杞1号和宁杞7号受到NaCl胁迫后,盐浓度在1.2和1.0 mol/L,枸杞幼苗达到耐盐阈值,在1.5 mol/L时达到耐盐极限值;Na2CO3胁迫后,盐浓度在0.2和0.1 mol/L幼苗达到耐盐阈值,在0.5和0.4 mol/L达到耐盐极限值;黑杞1号对于NaCl和 Na2CO3的耐受阈值要高于宁杞7号,Na2CO3对枸杞幼苗影响大于NaCl影响.