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Preliminary Study on Polyploid Induction of Xinjiang Lavender

LIAO Qing;Marhaba Wsman;SHA Hong;GAO Yan;GONG Song-wang;LIAO Zhi-li   

  • Received:2017-04-25 Revised:2017-04-25 Online:2017-04-25 Published:2017-04-25

新疆薰衣草多倍体诱导研究

廖晴;玛尔哈巴·吾斯满;沙红;高燕;龚松旺;廖志立   

  1. 新疆农业科学院园艺作物研究所,乌鲁木齐,830091;新疆霍城县农业技术推广站,新疆霍城,835200

Abstract: [Objective] The polyploid plants will be induced by tissue culture seedlings of C-197, which is the main cultivar of Xinjiang lavender, and the rapid propagation system of polyploid plants will be established, the research aims to explore the effective ways of Xinjiang lavender germplasm resources innovation technology and provide the technical support for the Xinjiang lavender industry.[Method]The optimal strains of C-197 tissue-cultured plantlets were taken as the testing material, polyploid plants were induced on polyploid induction medium MS+1 mg/L 6-BA+2; DMSO + different concentrations of colchicine.Different time treatments causing polyploidy in different concentrations of colchicines were compared, thus determining the suitable concentration and time for C-197 polyploid colchicine concentration and treatment time.[Result]Xinjiang lavender C-197 optimum strains of aseptic seedlings can make its seedlings to produce polyploid after 48-72 h explanted on induction medium MS+1 mg/L6-BA+2; DMSO +0.2;-0.4; colchicines.[Conclusion]Using the test method of inducing polyploid plants from tissue-cultured plantlets of optimal strains of C-197 can quickly realize the lavender polyploid plant new variety breeding, shorten the breeding period, improve and promote the Xinjiang lavender quality seedlings breeding rate and germplasm innovation work.

摘要: [目的]通过对新疆薰衣草主栽品种C-197优选优株的组培苗进行多倍体植株诱导,并对诱导成功的多倍体植株进行快繁体系的建立,探讨新疆薰衣草种质资源创新技术的有效途径,为新疆薰衣草产业提供种质资源创新技术支撑.[方法]采用新疆薰衣草C-197优选优株的组培苗为材料,接种至多倍体诱导培养基 MS+1 mg/L 6-BA+2; DMSO(二甲基亚砜)+不同浓度的秋水仙素中,进行多倍体诱导,比较在不同浓度的秋水仙素中,处理不同时间产生多倍体的差异,确定适合诱导新疆薰衣草C-197多倍体秋水仙素的浓度及处理时间.[结果]新疆薰衣草C-197优选优株的无菌苗,接种于MS+1 mg/L6-BA+2;二甲基亚砜+0.2;~0.4;秋水仙素的诱导培养基上处理48~72 h,均可使新疆薰衣草C-197的无菌苗产生多倍体.[结论]运用新疆薰衣草C-197多倍体诱导的试验方法,可快速实现对新疆薰衣草多倍体植株的新品种选育,缩短育种周期,有效提高和推进新疆薰衣草优质种苗的培育速率及种质创新.