Abstract: [Objective] In order to provide a scientific basis for the prevention and control of garlic root rot disease in Xinjiang, potential pathogens were isolated and identified from root garlic tissues.[Method]Using conventional tissue separation method for the separation and purification of pathogenic bacteria of the strains were identified by traditional morphological identification method combined with molecular biological methods.The method of in situ root injury was used to control the species and pathogenicity of pathogenic bacteria by contrast with Koch's postulate.[Result]64 isolates were purified from 3 batches root rot samples in Jimsar County, in which 61species were finally identified as the Fusarium spp.F.oxysporum, F.proliferatum, F.tricinctum, F.redolens, F.verticillioides, F.equiseti were involved in this disease.F.oxysporum and F.proliferatum could be isolated from all growth stages with frequency of 76.6; in total.In situ pathogenicity tests demonstrated that 5 typical strains could cause the infection of root rot at different levels, and the major pathogen was F.oxysporum, followed by F.proliferatum.[Conclusion]Different species of Fusarium spp.are involved in garlic root rot in Xinjiang.F.oxysporum was considered as the dominant pathogen because of its wide distribution, high frequency of isolation and strong pathogenicity.

摘要： [目的]研究新疆吉木萨尔大蒜种植区大蒜根腐病的病原菌.[方法]采用常规组织分离法分离纯化病原菌,用传统的形态学鉴定方法,结合分子生物学方法对菌株进行鉴定.用原位伤根法进行回接,对照科赫法则明确致病菌的种类及其致病力.[结果]在采集的3批次典型病害植株组织和根际土壤中分离得到64株分离物,其中61株分属于镰刀菌,分属于尖孢镰刀菌、层生镰刀菌、芳香镰刀菌、三线镰刀菌、轮状镰刀菌和木贼镰刀菌.尖孢镰刀菌和层生镰刀菌分离频率累计达到76.6;,且在采集的各个时期都有分离出现.选取典型的5株菌株进行回接测定,回接的5个镰刀菌种均表现出不同程度的致病性,其中尖孢镰刀菌是大蒜根腐病分离频率最高、且致病性最强的致病菌,其次为层生镰刀菌.[结论]新疆吉木萨尔地区大蒜根腐病是由镰刀菌属的真菌引起的,其中尖孢镰刀菌是致病的优势种.