]*>","")" /> Determination of Contents and Activities of Detoxification Enzymes of Helicoverpa armigera in Field Populations in Shihezi District
Xinjiang Agricultural Sciences

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Determination of Contents and Activities of Detoxification Enzymes of Helicoverpa armigera in Field Populations in Shihezi District

WANG Xiao-dong;WU Wan-feng;ZHANG Jian-hua;ZHAO Yi-ying   

  • Received:2012-12-25 Revised:2012-12-25 Online:2012-12-25 Published:2012-12-25

石河子地区棉田棉铃虫种群体内相关酶活及含量的测定

汪小东;武万锋;张建华;赵伊英   

  1. 石河子大学农学院,新疆石河子,832003





摘要： [目的]测定石河子地区棉铃虫体内羧酸酯酶、磷酸酯酶、谷胱甘肽-S-转移酶以及多功能氧化酶(MFOs)四类主要解毒代谢酶的含量及活性,并与敏感品系棉铃虫进行比较,为棉铃虫抗药性研究提供实验依据.[方法]利用紫外分光光度计比色法测定酶源蛋白含量及比活力.[结果]石河子地区棉铃虫体内的MFOs的酶原蛋白含量及比活力均高于敏感品系棉铃虫且差异显著,其酶源蛋白含量分别为105.943 167、96.158 47μ头,比活力(0.485 3 ±0.002 1)、(0.352 3 ±0.001 4)μM/μg·30 min,其余三种酶在两品系间差异不显著.[结论]MFOs活性的增加是石河子地区棉铃虫抗药性增强重要因素之一.