]*>","")" /> The Cloning and Expression of gdh Gene from Corynebacterium glutamicum in Brevibacterium flavum C -11
Xinjiang Agricultural Sciences

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The Cloning and Expression of gdh Gene from Corynebacterium glutamicum in Brevibacterium flavum C -11

  • Received:2011-09-25 Revised:2011-09-25 Online:2011-09-25 Published:2011-09-25

谷氨酸棒状杆菌gdh基因在黄色短杆菌C-11中的克隆及表达

王宁;包慧芳;杨慧;王炜   

  1. 新疆农业科学院微生物应用研究所,乌鲁木齐,830091





摘要： [目的]研究谷氨酸脱氢酶的表达对L-丝氨酸产率及发酵效率的影响.[方法]从谷氨酸棒杆菌模式菌株C.glutamicum ATCC39135中克隆出gdh基因,以大肠杆菌-黄色短杆菌穿梭表达载体pEC7为基础,构建重组质粒pEC7G,并转化黄色短杆菌B.flavum C -11,获得重组菌株C-11G.[结果]重组菌株C-11G的GDH酶活力提高32.7;,产酸率为15.28 g/L,比原宿主菌提高了33.80;.[结论]成功构建重组菌株C-11G,所克隆的gdh基因有较高表达活性,并对L-丝氨酸产量的提高有促进作用.