Abstract: [Objective] This study aims to clone Carpomya vesuviana Costa odorant binding protein(CvcoOBP)and analyze expression profile of CvcoOBP in order to lay the foundation of researching CvcoOBP in Carpomya vesuviana Costa.[Method]Based on transcriptome sequencing of cephalic RNA,the study has analyzed CvcoOBP according to alignment and phylogenetic analysis.The research was also conducted into the tissue specific expression of CvcoOBP by semi-quantitative reverse transcription and polymerase chain reaction(sqRT-PCR).[Result]The study has obtained seven CvcoOBP that had been named respectively as CvcoOBP1-7(GenBank accession number: KU975053,KU975054,KU975055,KX059394,KU975056,KX059395 and KX059396).According to amino acid alignment,CvcoOBP1,CvcoOBP3,CvcoOBP4,CvcoOBP6 and CvcoOBP7 had been proved to be a family of classic OBP,which contained six highly conversed cysteine residues.CvcoOBP2 and CvcoOBP5 belonged to a family of "Minus-C",which had only four highly conversed cysteine residues.Phylogenetic analysis showed that CvcoOBP1-7 had close relationships with different OBPs.Finally,sqRT-PCR revealed that CvcoOBP1-7 could be highly expressed in abdomen,head and thorax.CvcoOBP1 and CvcoOBP2 could be also expressed in wings with relatively low expression.CvcoOBP1,CvcoOBP5 and CvcoOBP6 were also found in legs.[Conclusion]For the first time,the study obtained and analyzed seven CvcoOBP.Different CvcoOBP are various in terms of expression sites and expression quantity.The CvcoOBP1-6 separately belongs to OBP56g,OBP8a,OBP83a,OBP56h,OBP99c and OBP83ef type.

摘要： [目的]克隆枣实蝇Carpomya vesuviana Costa气味结合蛋白(odorant binding protein,CvcoOBP)基因,对其表达谱进行分析,为枣实蝇气味结合蛋白的深入研究奠定基础.[方法]对枣实蝇头部RNA进行转录组测序,通过蛋白序列比对以及构建系统进化树分析枣实蝇OBP序列,运用半定量反转录-聚合酶链反应(semi-quantitative reverse transcription and polymerase chain reaction,sqRT-PCR)方法对获得的CvcoOBP序列进行组织表达特异性研究.[结果]转录组测序后共获得7个OBP,命名为(GenBank登录号分别为:KU975053、KU975054、KU975055、KX059394、KU975056、KX059395、KX059396).对CvcoOBP1-7进行蛋白序列比对发现,CvcoOBP1、CvcoOBP3、CvcoOBP4、CvcoOBP6、CvcoOBP7是典型的OBP家族成员,具有6个高度保守的半胱氨酸残基;CvcoOBP2和CvcoOBP5属于"Minus-C"家族成员,具有4个高度保守的半胱氨酸残基.系统进化分析结果显示,CvcoOBP1-7分别与不同的OBP有较近的进化关系.半定量PCR检测结果发现,CvcoOBP1-7在枣实蝇腹部、头部及胸部都有表达,且表达量较高;CvcoOBP1、CvcoOBP2在翅中有表达,但表达量相对较低;CvcoOBP1、CvcoOBP5、CvcoOBP6在足中也有表达,但表达量相对较低.[结论]获得并分析了7个CvcoOBP,不同CvcoOBP表达部位和表达量各不相同;CvcoOBP1-6分别属于OBP56g,OBP8a,OBP83a,OBP56h,OBP99c,OBP83ef类型.