]*>","")" /> Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis
Xinjiang Agricultural Sciences

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Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis

HAN Hui;WU Xiao-hu;LI Yi;LI Ke-mei;ZHAO Li   

  • Received:2011-07-25 Revised:2011-07-25 Online:2011-07-25 Published:2011-07-25

紫外线诱变选育苜蓿叶象甲高毒力白僵菌

韩慧;吴小虎;李益;李克梅;赵莉   

  1. 新疆农业大学农学院,新疆草地资源与生态实验室,乌鲁木齐830052





摘要： [目的]选育出对苜蓿叶象甲高毒力的白僵菌.[方法]利用紫外线对前期筛选出的白僵菌菌株照射10和30min进行诱变,选出高几丁质酶突变株,再用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫进行毒力测定.[结果]用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫室内毒力测定:处理9d后,诱导菌株Bb- 10- 12校正死亡率为100;,Bb - 30 - 14校正死亡率为77.78;,而朱经紫外线照射的野生型菌株校正死亡率为62.97;.[结论]通过室内毒力测定法,获得了经10 min紫外线照射诱变对苜蓿叶象甲更高毒力的1株白僵菌菌株.