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CONG Hua;WANG Hong-fei;ZHANG Yan-feng;YANG Yong-liang;Tatsuya Ikeda;Kanenori Takata;Tsukasa Nagamine
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丛花;王宏飞;章艳凤;严勇亮;池田达哉;高田兼则;长峰司
摘要: [目的]籽粒硬度是重要的小麦品质性状,了解新生疆小麦地方品种籽粒硬度的变异类型和分布,为新疆小麦品质育种提供遗传信息.[方法]利用单籽粒硬度仪(SKCS)和PCR扩增、酶切及DNA测序技术,结合改良的Friabilin提取及电泳分析方法,对105份新疆小麦地方品种的籽粒硬度及Pina和Pint等位基因进行研究.[结果]新疆小麦地方品种以硬质类型为主,平均硬度值为63.5.新疆小麦地方品种Puroindoline基因共有9种突变型,分别是Pina-D1a/Pinb-D1a,Pina-D1a/Pinb-Dlb,Pina-D1a/Pint-D1p,Pina-D1k ()/,Pina-D1l/Pinb-D1a,Pina-D1r/Pinb-D1a,Pina-D1a/Pinb-D1ab,Pina-D1a/Pint-Dlac和Pina-D1b/Pinb-D1p,其中Pina-D1 r/Pinb-D1a为新发现的突变型.新疆小麦地方品种Puroindoline基因型以Pina-D1a/Pinb-D1p为主导类型,共有62份,占59.1;;其Pina-D1k()/和Pina-D1 a/Pint-D1ab两种突变型与其地理来源和生态型密切相关,主要分布在南疆冬小麦中.Pina-D1a/Pinb-D1a(野生型)的硬度值显著低于突变型,Pina-Dlk()/的硬度值最高,但Pins-D1k ()/ ,Pina-D1a/Pinb-D1p和Pina-D1a/Pinb-D1ab的硬度值差异不显著.[结论]新疆小麦地方品种籽粒硬度及其Puroindoline等位变异和分布信息能为新疆小麦品质改良和引种提供理论依据.
CONG Hua;WANG Hong-fei;ZHANG Yan-feng;YANG Yong-liang;Tatsuya Ikeda;Kanenori Takata;Tsukasa Nagamine. Identification of the Allelic Variation of Puroindoline Alleles in Xinjiang Wheat Landraces[J]. .
丛花;王宏飞;章艳凤;严勇亮;池田达哉;高田兼则;长峰司. 新疆小麦地方品种籽粒硬度及其Puroindoline 基因等位变异研究[J]. .
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http://www.xjnykx.com/EN/Y2011/V48/I6/1056