]*>","")" /> Study on the Nest-PCR to Detect the Albugo tragopogi (Persoon)Schruter var Helianthi Novotlenova
Xinjiang Agricultural Sciences

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Study on the Nest-PCR to Detect the Albugo tragopogi (Persoon)Schruter var Helianthi Novotlenova

LIU Bin;ZHANG Xiang-lin;WANG Chong;QIAN Yi-ke;CHEN Yan   

  • Received:2011-05-25 Revised:2011-05-25 Online:2011-05-25 Published:2011-05-25

向日葵白锈病菌巢式PCR检测方法的研究

刘彬;张祥林;王种;乾义柯;陈燕   

  1. 新疆出入境检验检疫局,乌鲁木齐830063;新疆农业大学农学院,乌鲁木齐830052;新疆出入境检验检疫局,乌鲁木齐,830063





摘要： [目的]建立向日葵白锈病菌(Albugo tragopogi(Persoon)Schruter var helianthi Novotlenova)的巢式PCR检测方法.[方法]用通用引物NL1/NL4对采自伊犁的向日葵白锈病菌进行PCR扩增、克隆、酶切和测序,设计该病菌的特异性引物,用巢式PCR法检测供试样品.[结果]序列测定和比对结果表明,该病菌的核酸序列与GeneBank公布的向日葵白锈病菌核酸序列同源率为97;.针对该段基因设计向日葵白锈病菌的特异性引物ATHP3/ATHP4,用巢式PCR法对供试样品进行扩增,扩增片段为370bp.[结论]成功建立了向日葵白锈病菌巢式PCR检测方法.