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NI Jian-hong;LIU Chang-bin;ZHANG-bin;Graham W Kay
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倪建宏;刘长彬;张宾;Graham W Kay
摘要: [目的]新鲜精液在离开生殖腺后存活时间很短.相关研究表明副睾液为精子的存活提供了特殊的环境.试验探讨和研究牛附睾精子在体外模拟附睾液中的存活试验的各因素的影响.[方法]试验对公牛附睾头部和尾部的摩尔渗透压和蛋白质浓度进行了分析,牛附睾尾部精子在不同蛋白浓度的CEP-2溶液中,在4℃条件下孵育120h.每24h做一次精子活力检测.[结果]5 d后精子活力仍然超过60;.附睾头、尾部的渗透压分别为(287.0±13.7)mOsm和(310.8±17.0)mOsm.蛋白浓度分别为(37.43±12.55)mg/mL和(50.58±11.08)mg/mL.[结论]附睾尾精子在蛋白浓度为40mg/mL,渗透压为345 mOsm时,精子存活率最高.
NI Jian-hong;LIU Chang-bin;ZHANG-bin;Graham W Kay. Medium-term Survival of Bovine Caudal Epididymal Sperm under Conditions Based on Epididymal Fluid Characteristics[J]. .
倪建宏;刘长彬;张宾;Graham W Kay. 牛附睾和附睾液分析与牛附睾尾精子在模拟附睾液中存活试验[J]. .
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http://www.xjnykx.com/EN/Y2011/V48/I5/937