]*>","")" /> Determination of Swainsonine Content in Oxytrpis glabra DC in South Xinjiang by Internal Standard Gas Chromatography
Xinjiang Agricultural Sciences

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Determination of Swainsonine Content in Oxytrpis glabra DC in South Xinjiang by Internal Standard Gas Chromatography

WANG Shuai;CHEN Gen-yuan;HU Jian-jun;MA Chun-hui   

  • Received:2011-04-25 Revised:2011-04-25 Online:2011-04-25 Published:2011-04-25

气相色谱内标法测定南疆地区小花棘豆中苦马豆素含量

王帅;陈根元;胡建军;马春晖   

  1. 塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300





摘要： [目的]建立以甲基-α-D-吡喃甘露糖苷(me-Gal)为内标,用气相色谱法测定小花棘豆中苦马豆素含量的方法.[方法]在柱温200℃、进样口温度300℃、氢火焰离子化检测器温度280℃、高纯氮气载气压力200 kPa条件下,对苦马豆素(SW)进行气相色谱分析.[结果]在0.375～6 mg/mL SW质量浓度范围内对SW和me-Gal峰面积比值的线性关系良好,加标平均回收率为102.18;,RSD=1.724;(n=5).小花棘豆中各部位苦马豆素含量为:种子(58.09±0.48)mg/kg;叶(21.81±0.58)mg/kg;全株(19.14±0.16)mg/kg;茎(12.30±0.23 mg/kg).[结论]该方法结果准确,可用于小花棘豆中苦马豆素含量的测定.