Abstract: [Objective] [Method] This study aims to explore the effects of artificial aging on seed vigor,starch granule,starch mass fraction and analyze the activity and endogenous hormones in the endosperm of barley seeds with high temperature and high humidity artificial aging treatment in the early stage of germination.[Result]The results showed that:The germination indexes and embryo vitality of 4 varieties decreased gradually with increasing aging time.Artificial aging increased the small starch granules and reduced large starch granules of endosperm in the germination 72 h.In addition to that,artificial aging increased starch mass fraction during the germination,reduced the activity of α-amylase and β-amylase of all barley and there were significant differences between the aging 15 d and the control.Content of GA3 was lower than that of CK in the germination 24 h but higher than that of the CK in the germination 48 to 72 h.However,contents of ABA were always higher than those of the CK in the all germination process.[Conclusion] The balance between GA3 and ABA was broken by aging,which resulted in the decrease of amylase activity and the slow-down of starch decomposition,so then barley seed vigor was decreased and the germination inhibited.

摘要： [目的]研究老化处理对大麦种子活力及其萌发早期胚乳淀粉粒类型、淀粉含量、淀粉酶活性及内源激素的影响.[方法]采用高温高湿的人工老化处理方法.[结果]人工老化使4个大麦品种的发芽特性指标和种胚活力均逐渐降低.萌发72 h的种子胚乳中小淀粉粒数目增加,大淀粉粒数目减少,淀粉质量分数增加.随着老化时间的延长,α-淀粉酶、β-淀粉酶和极限糊精酶活性均呈降低的趋势,且老化15 d的种子淀粉酶活性显著低于对照;萌发24h,GA3含量显著低于对照,萌发48～72 h,GA3含量均高于对照.ABA含量在萌发过程中均高于对照.[结论]人工老化打破了萌发过程中GA3和ABA间的平衡,导致淀粉酶活性降低,淀粉分解减缓,进而使大麦种子活力降低,萌发受到抑制.