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LI Hai-Qiang;WANG Dong-Mei;XU Yao;ZHANG Yong-Jun;DING Rui-Feng;LI Hao-Bin;WANG Fei;Ahtam·Uwayis;LIU Jian
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李海强;王冬梅;徐遥;张永军;丁瑞丰;李号宾;汪飞;阿克旦·吾外士;刘建
摘要: [目的]研究转Bt基因棉花对多异瓢虫生长发育及中肠组织的变化.[方法]用取食转Bt-Cry 1Ab/Ac基因棉棉蚜喂饲多异瓢虫,初步研究了取食转Bt-CryAb/Ac基因棉棉蚜对多异瓢虫的影响.利用透射电镜观察取食转Bt-Cry1Ab/Ac基因花粉和取食对照花粉的多异瓢虫中肠组织的变化.用ELISA检测方法在取食转Bt-Cry1Ab/Ac基因花粉的多异瓢虫体Bt毒蛋白进行了检测.[结果]多异瓢虫Hippodamia variegata取食棉蚜后各个虫态均无显著差异;利用透射电镜观察取食转Bt-Cry1Ab/Ac基因花粉和取食对照花粉的多异瓢虫中肠组织,发现其微绒毛、线粒体、内质网均无显著变化;用ELISA检测方法在取食转Bt-Cry1Ab/Ac基因花粉的多异瓢虫体内没有检测到Bt毒蛋白.[结论]取食转Bt-Cry1Ab/Ac基因棉棉蚜和花粉对多异瓢虫生长发育没有明显影响.
LI Hai-Qiang;WANG Dong-Mei;XU Yao;ZHANG Yong-Jun;DING Rui-Feng;LI Hao-Bin;WANG Fei;Ahtam·Uwayis;LIU Jian. Effects of Transgenic Bt-Cry 1Ab/Ac Cotton on the Growth and Development and Pathological Changes in Midgut Tissues of Hippodamia[J]. .
李海强;王冬梅;徐遥;张永军;丁瑞丰;李号宾;汪飞;阿克旦·吾外士;刘建. 转Bt基因棉对多异瓢虫生长发育及中肠组织变化研究[J]. .
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http://www.xjnykx.com/EN/Y2011/V48/I3/511