Abstract: [Objective] To study the correlation of stone cell content and POD4 gene expression in rough skin pear and normal skin fruit of mature Korla Fragrant Pear in the hope of providing molecular basis for improving the fruit quality.[Method] Rough skin fruit and normal skin fruit of Korla Fragrant Pear during the mature period were used as materials,and the firmness and the content of stone cell were determinated.Total RNA was extracted from fragrant pear fruit pulp,quantification expression of POD4 gene was determinated by Real-time fluorescent quantitative polymerase chain reaction (qRT PCR),the correlation of the firmness and the content of stone cell and quantification expression of POD4 gene were analyzed.[Result] The content of fruit stone cell was between 0.4 and 0.52 gram,pear firmness was between 245.22 and 314.71 PA and quantification expression of POD4 gene in mature pear was between 106.89 and 743.29.[Conclusion] The content of stone cell in rough skin fruit is 1.3 times than the normal skin fruit,the hardness was 1.28 times,POD4 gene expression was 6.59 times.The correlation between the content of stone cell and the quantification expression of POD4 gene was significantly positive (r =0.823),and correlation between the content of stone cell and the fruit hardness was positive (r =0.783).The correlation between quantification expression of POD4 and fruit firmness was positive (r =0.770).Therefore,the level of expression of POD gene can be used as a reference index for the identification of the content of stone cell.

摘要： [目的]研究成熟期库尔勒香梨粗皮果和正常果的石细胞含量与POD4基因即时表达量的相关性,为改良香梨果实品质提供分子依据.[方法]以成熟期库尔勒香梨粗皮果和正常果为试材,测定香梨果实的硬度和石细胞含量.提取香梨果肉总RNA,用qRT-PCR(Real-time fluorescent quantitative polymerase chain reaction)方法测定POD4(peroxidase4)基因的即时表达量,分析硬度和石细胞含量与POD4基因表达的相关性.[结果]库尔勒香梨石细胞含量在0.4 ～0.52 g;硬度在245.22 ～314.71 kg/cm2;POD4基因在成熟果实中的即时表达量在106.89 ～743.29.[结论]粗皮果石细胞含量是正常果的1.3倍,硬度是正常果的1.28倍,POD4基因的即时表达量是正常果的6.59倍.石细胞含量与POD4基因表达量成显著正相关(r=0.823),与果实硬度存在正相关(r =0.783),POD4基因表达量与果实硬度存在正相关(r=0.770).POD4基因表达差异与形成两种表型库尔勒香梨果实成正相关.