Abstract: [Objective] To obtain PgANS,PgCHS and PgACT genes in Kashgar pomegranate kernels and analyze their sequence.[Method] Xinjiang pomegranate in Kashgar was selected as the materials.The total RNA of pomegranate Kernels was extracted with Phenol-chloroform.The first cDNA was synthesized with TIANScript kit,and amplified to cDNA by RT-PCR using special primer.The pMD19-T vector linked with the cDNA fragment was transformed into Escherichia coli DH5α,and the positive clone was sequenced.[Result] The fragment of PgANS was 474bp,GenBank accession number was KT779433.Compared with Punica granatum L.which the accession numbers were KF841619,GU376749 on NCBI,the PgANS homology of nucleotide sequence was 99;.At the same time,the sequence analysis demonstrated that the deduced nucleotide sequence shared 85;,79; and 76; homology with that of Ipomoea batatas (GU598212),Morus alba (JF499385) and Fragariax ananassa (AY695817);the fragment of PgCHS was 140bp and the PgACT was 148bp.Compared with Punica granatum L.(JF747148,GU376750) on NCBI,the PgCHS homology of nucleotide sequence was 99;,and the PgACT was 93;.[Conclusion] The PgANS,PgCHS and PgACT genes have been obtained,which has laid the foundation for further studying the influence of storage conditions on the anthocyanins of Punica granatum L.kernels.

摘要： [目的]获得石榴果实PgANS、PgCHS和PgACT基因,并对这三个基因进行序列分析.[方法]以喀什甜石榴为试材,通过酚-氯仿法提取籽粒中的总RNA,用TIANScript cDNA试剂盒反转录合成第一链cDNA,并用特异引物对其RT-PCR扩增,将得到的cDNA片段连接到PMD19-T载体上,然后将该载体转到DHScα,并对阳性克隆进行序列分析.[结果]PgANS基因片段长度为474 bp,Genbank登录号为KT779433,与NCBI数据库中KF841619、GU376749两种石榴核苷酸的同源性均为99;,与甘薯核苷酸(GU59821)、白桑核苷酸(JF499385)、草莓核苷酸(AY695817)的同源性分别为85;、79;、76;;PgCHS和PgACT基因片段长度分别为140 bp和148 bp,分别与NCBI数据库中JF747148、GU376750两种石榴核苷酸同源性为99;和93;.[结论]获得PgANS、PgCHS和PgACT基因片段,为进一步研究贮藏条件对石榴籽粒花色苷的影响奠定了基础.