Abstract: [Objective] This project aims to isolate and screen 70 actinomycetes strains from the high radiation soil of Xinjiang by antagonistic effect of 8 kinds of pathogenic bacteria such as the Valsamali Miyabe et Yamada.[Method] By the plate confrontation culture method,and through initial screening and repeated screening of antagonistic effect of 8 kinds of pathogenic bacteria,and according to the morphological characteristics of the strains of CH10,the physiological and biochemical characteristics and 16S rDNA sequence went through identification and analysis.[Result] The results showed that 8 kinds of important pathogens of Xinjiang crops,such as the Valsa mali Miyabe et Yamada,were screened out from 70 kinds of actinomycetes,which had good antagonistic effect called CH10.[Conclusion] Strain CH10 belongs to the genus streptomyces and it has good antagonistic effect on the Valsa mali Miyabe et Yamada,which has provided a foundation for the biological control of the disease.

摘要： [目的]从新疆高放射土壤中分离和筛选出对苹果树腐烂病等有拮抗作用的放线菌,并对其进行鉴定.[方法]采用对峙培养法通过初筛、复筛测定拮抗菌对8种病原菌的拮抗效果,根据筛选出菌株CH10的形态特征、生理生化特性及16S rDNA序列对其进行鉴定.[结果]从70种放线菌中筛选出对苹果树腐烂病等8种新疆作物重要病原菌都具有良好拮抗效果的CH10.[结论]菌株CH10属于链霉菌属,对苹果树腐烂病菌等有良好的拈抗效果,为该病害的生物防治提供研究基础.