Abstract: [Objective] In this study,the project aims to analyze the association of DMRT3 gene polymorphism with gait trait in trotting type of 103 individuals Yili horse.[Method] Genetic polymorphism of DMRT3 gene was detected by PCR-RFLP and DNA sequencing technology.[Result] The result showed that there was 1 mutation in DMRT3 gene and there were 3 genotypes:CC,CA and AA.The SNP g.22999655 C ＞ A was identified in DMRT3 gene by sequencing,and it caused the premature stop of the encoded acid.Statistical results indicated that the SNP was significantly associated with speed in Yili horse (P ＜ 0.05).[Conclusion] The mutation might be acted as an crucial DNA genetic marker for trotting performance traits selection in Yili horse.

摘要： [目的]以103匹伊犁马(速步型)为研究对象,研究伊犁马DMRT3基因多态性与步法性状之问的相关性.[方法]运用PCR-RFLP方法对伊犁马DMRT3基因进行多态性分析.[结果]该基因在伊犁马上存在3种基因型,分别为CC、CA和AA,测序结果显示:DMRT3的碱基突变位置为g.22999655C ＞A,该突变导致氨基酸编码提前终止,经关联分析,该突变对伊犁马速度有显著影响(P＜0.05).[结论]伊犁马DMRT3基因突变与速度之间存在关联性,且可能是伊犁马步法性状的重要位点.