Abstract: [Objective and Method]The gene chip is a DNA analysis technology of high throughput and high efficiency which has been widely used in analysis of resistant function.In this study, using Affymetrix Arabidopsis whole-genome expression gene chip, the differential expression analysis of gene was carried out with the transgenic A. thaliana .[Result]In the transgenic Arabidopsis tissues under salt stress, 394 co-expression genes were selected from the four groups, they accounted for 1.64; of the total number of genes, in which there were 177 up-regulated expression genes and 217 down-regulated expression genes. These genes were initially divided into 12 gene categories, including response to abiotic or biotic stimuli, response to stress , electron transport or energy pathways, signal transduction and transcription, and so on. Our results show that the process of plant salt-tolerant is a multi-gene changed complex process that involves many changes of variety related genes.[Conclusion]The results of comparison scatters and cluster analysis illuminate that the salt tolerance of transgenic Arabidopsis cgNHX1, cgNHX1dc, osNHX1 and osNHX1dc have successively weakened.Our study will contribute to find the differences between halophytic and glycophytic species in the mechanism of plant salt tolerance and the function analysis of hydrophilic C-terminal, provide a favorable evidence for the study of the mechanism of plant salt-tolerance between halophytic and glycophytic species, and offer new methodology in study the mechanism for salt-tolerant plants, provide a basis for practical applications of salt-tolerant mechanism in plants.

摘要： [目的]基因芯片作为高通量、高效率的DNA分析技术,已被广泛地用于植物抗逆性功能基因的分析之中.为探讨盐生植物和甜土植物NHX1基因调控盐应答过程和亲水性C端的功能研究提供借鉴,进一步揭示盐生植物和甜土植物耐盐机制的差异,为植物耐盐机理的实际应用提供依据.[方法]利用Affymetrix拟南芥表达谱芯片对过表达盐生植物灰绿藜和甜土植物水稻NHX1基因及缺失部分C端基因的转基因拟南芥进行基因表达差异的分析.[结果]在盐胁迫的转基因拟南芥组织中,筛选出在四组转基因型拟南芥中共同差异表达的基因有394条,占筛选基因总数的1.64;,其中上调表达的基因有177条,下调表达的基因有217条.将这些差异基因初步分为12类, 包括非生物性与生物性刺激的应答过程、胁迫应答过程、电子转移和能量途径、信号转导和转录等功能类别.[结论]表明植物耐盐过程是一个多基因参与的复杂的变化过程, 涉及到许多相关基因的变化.联系两两比较散点图和聚类分析结果从理论上说明,转基因拟南芥cgNHX1,cgNHX1dc,osNHX1和osNHX1dc耐盐性有依次减弱的趋势.