Abstract: [Objective and Method]In this study 6 genotypes of upland cotton were selected for comparison of their regenerability,including 2 cultivars from Yellow River valley, 3 cultivars from Xinjiang,using a highly responsible genotypes Coker 312 as a material. [Result]Most of the genotypes showed high regenerability under the IBA/KT hormone regime.[Conclusion]Under the optimized culture procedure, most of the genotypes collected from the two planting areas showed the similar regeneable abilities.The total number of embryos and frequency of embryo germination of "XLZ15"was higher for cotton transformation. This study will improve the transgenic efficiency in Xinjiang.

摘要： [目的]比较新疆和黄河流域两生态区棉花品种的体细胞再生能力,建立高效棉花体细胞再生体系.[方法]以新疆棉区的新陆中15号、新陆中18号、系9;黄河流域棉区的中棉所35号、中棉所44号及对照品种柯字312为研究对象,优化棉花体细胞胚胎发生的相关条件参数.[结果]在IBA+KT的激素组合下,6个棉花品种再生了5个.[结论]新疆棉区和黄河流域棉花品种的植株再生潜力没有明显差别,但新疆棉花品种体细胞胚胎发生所需的时间长.新陆中15号的愈伤组织分化率及胚胎萌发率都比较高,具备作为棉花优良遗传转化受体的基本特征,目前已经用于农杆菌介导的转基因试验,为新疆棉花组织培养及转基因育种工作提供了重要基础材料.