Abstract: [Objective and Method]In this experiment,the upland cotton resistant parent Liaomian 18 and sensitive parent Junmian 1 and their F_2 generation were used for identification of the molecular markers linked to disease-resistance gene of cotton by SRAP-BSA,[Result]88 pairs of primers were used to amplify the parents gene and two gene pools. When 3 pairs of primers were found in parents and two pools,three pairs of primers were screened from 88 paris of SRAP primers.[Conclusion]The fragment existed in the resistant plants and F population,they were indicated that the SRAP marker were closely linked to disease-resistance gene of cotton.

摘要： [目的]以高抗黄萎病品种辽棉18号和高感黄萎病品种军棉1号及其杂交后代F2为材料,筛选棉花抗病基因.[方法]采用分离群体分组分析(Bulked Segregate Analysis,BSA)法,对棉花抗病基因进行SRAP分析.[结果]在88对SRAP引物中筛选出3对引物在两亲本及抗、感基因池中均能扩增出稳定的差异条带,命名为S_(6-6)、S_(6-10)、S_(8-8).[结论]通过F2代个体验证,抗病个体均能扩增出此差异条带,而感病个体中不能扩增出此差异条带,证明这三对引物是与棉花抗病基因紧密连锁的SRAP分子标记.