Abstract: [Objective]For protecting and utilizing Tricholoma mongolicum Imai.,using liquid culture to grasp its nutritional characteristics,[Method]taking the Tricholoma mongolicum from Bayanbulak as the material for shake cultivation,the biomass,characteristics and culture time are studied to explain its nutritional characteristics and suitable culture condition.[Result]The result indicates that the mycelium can be cultured in the liquid medium with a certain time and the best medium formulation is corn grits 5;,glucose 2;,KH_2PO_4 0.1;,MgSO_4 0.05;,soybean 0.1; and pH 6.5～7.0. It has enough dissolved oxygen and a more stable biomass when there is 40; liquid medium in the bottle and cultured at the speed 125 r/min,the biomass is 0.684 8 g/100 mL and the mycelium age is 21 d.[Conclusion]It has a poor adaptation ability when growing both in the potato medium and the rice bran medium.

摘要： [目的]掌握新疆巴音布鲁克草原蒙古口蘑的营养代谢特点,为进一步开展蒙古口蘑资源利用提供理论指导.[方法]以巴音布鲁克草原蒙古口蘑为材料,采用液体培养技术,通过研究生物量、菌球特征、培养时间等内容,阐明蒙古口蘑液体培养的营养代谢特性以及适宜的液体培养条件.[结果]液体培养基配方为玉米糁5;,葡萄糖2;,磷酸二氢钾0.1;,硫酸镁0.05;,黄豆0.1;,pH 6.5～7.0时蒙古口蘑生物量较稳定,生物量干重达0.684 8 g/100 mL,菌龄为21d.培养基装量以摇瓶体积的40;为宜,转速125 r/min,溶解氧满足需要.[结论]巴音布鲁克蒙古口蘑对不同营养物质的代谢能力不同、培养时间亦不同.蒙古口蘑对马铃薯配方和米糠配方的营养代谢能力较差.