Abstract: [Objective and Method]Through the screening and orthogonal design experiments of the carbon, nitrogen and trace elements, [Result]the most suitable components of medium for strain's fermentation are found to be as follows (g/L): sucrose 60, peptone 30, NaCl 30, K_2HPO_4 2, MgSO_4 1, FeSO_4 0.02, MnSO_4 0.01. The research on fermental condition indicate that the optimal initial pH of fermentation is 5.5, the optimal temperature is 35℃,the optimal volume of liquid medium is 10 mL in 250 mL conical bottle. [Conclusion]The strain TS-Ⅱ's living barteria has reached 6.58×10~(10) CFU/mL after the optimization of the components of medium and cultivating condition.

摘要： [目的]地衣芽孢杆菌规模化发展.[方法]采用碳、氮源及微量元素的筛选实验和正交实验.[结果]确定地衣芽孢杆菌TS-Ⅱ菌株发酵培养基的最佳组分为(g/L):蔗糖60、蛋白胨30、NaCl 30 、K2HPO4 2、MgSO4 1、FeSO4 0.02、MnSO4 0.01.对发酵条件进行了研究,发酵的最适初始pH值为5.5,最适培养温度为35℃,最适装液量为10 mL(250 mL三角瓶).[结论]通过优化培养基组分和培养条件后,菌株TS-Ⅱ活菌数达到了6.58×10~(10) CFU/mL,为以后的工业化生产奠定基础.