Abstract: [Objective]A pair of primers were designed according to the three antifreezing proteins (AFP) gene of Ammopiptanthus mongolicus (Genbank accession number is AY590122, AY843521, and AY843522, respectively). [Method]Total RNA of Ammopiptanthus nanus was extracted using Trizol, which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.[Result]The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity (98.15;) to the AFP gene of Ammopiptanthus mongolicus.[Conclusion]The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus

摘要： [目的]从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物.[方法]以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域.[结果]序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15;.[结论]得到新疆沙冬青的抗冻蛋白的保守区域.