]*>","")" /> Identification on Molecule Biology of A Lactic Acid Bacteria and the Test of Cholesterol Degradation from Koumiss

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Identification on Molecule Biology of A Lactic Acid Bacteria and the Test of Cholesterol Degradation from Koumiss

CHEN Yang;YAO Xin-kui;PAN Dao-dong;ZENG Xiao-qun   

  • Received:2009-05-25 Revised:2009-05-25 Online:2009-05-25 Published:2009-05-25

一株酸马奶乳酸菌分子生物学鉴定及降解胆固醇试验研究

陈阳;姚新奎;潘道东;曾小群   

  1. 新疆农业大学动物科学学院,乌鲁木齐,830052;南京师范大学国家乳品加工技术研发分中心,南京,210097

摘要: 从新疆伊犁农牧民传统发酵的酸马奶中分离筛选到呈革兰氏染色阳性,秆状,编号为H-1的菌株.通过对此菌株进行形态观察和普通生理分析,并测定该菌株降解胆固醇的活力,通过16S r DNA序列分析表明,分离菌株H-1与Lactobacillus casei具有100;相似性,说明此菌株即为干酪乳杆菌.使用ClustalX1.8程序和Mega2程序将该菌株与报道的相关乳酸菌进行系统发育分析,揭示了16S r DNA序列分析在菌株鉴定的正确性.