]*>","")" /> Gene difF Cloning and Sequence Analysis on Cytochrone b5 Protein of Petunia hybrid Strain
Xinjiang Agricultural Sciences

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Gene difF Cloning and Sequence Analysis on Cytochrone b5 Protein of Petunia hybrid Strain

LI Ji-lian;SI Ai-jun;ZHU Jian -bo;DENG Fu-jun   

  • Received:2009-05-25 Revised:2009-05-25 Online:2009-05-25 Published:2009-05-25

矮牵牛细胞色素b5蛋白编码基因difF的克隆及序列分析

李吉莲;司爱君;祝建波;邓福军   

  1. 石河子大学生命科学学院,新疆石河子,832003;新疆农垦科学院棉花所,新疆石河子,832000;石河子大学生命科学学院,新疆石河子,832003;新疆农垦科学院棉花所,新疆石河子,832000





摘要： 以紫红色、蓝紫色矮牵牛(Petunia hybrida)的花瓣为材料,提取总RNA,用Oligo(dT)作为引物反转录合成cDNA第一链:以此为模板,用Genebank上的矮牵牛细胞色素b5蛋白DIF-F(difF)的mRNA序列设计成的引物进行PCR扩增,扩增到一条约450 bp的片段,将此片段克隆到pGM-T载体上.对重组克隆进行序列分析,结果表明所克隆到的矮牵牛细胞色素b5蛋白的cDNA的编码区含有450个核苷酸,编码149个氨基酸残基;其核苷酸及氨基酸的序列与Genebank上的同源性分别达到99.93;和100;.