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HAN Hai-xia;YANG Xiao-jun;Ayiguli·Aihemaiti;WANG Ying;ZHU Jin-fang;BAO Xiao-wei;MA Juan
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韩海霞;杨晓君;阿依古丽·艾合买提;王颖;朱金芳;包晓玮;马娟
摘要: 为了建立新疆巴旦杏中苦杏仁苷的HPLC定量测定方法,采用乙醇回流提取法提取苦杏仁苷,以 Zirchrom C18色谱柱(250 mm×4.6 mm,10 μm)为固定相,甲醇∶水∶冰醋酸(20∶80∶0.05)为流动相,流速为1.0 mL/min,检测波长为210 nm,柱温为35℃.测定结果显示,苦杏仁苷在20.0~70.0 μg/mL成良好的线性关系,r=0.999 9 ,日内、日间RSD均为0.59;(n=5),平均回收率99.6;(n=6).研究表明,该方法灵敏度高,结果准确,重现性好,方法简便可行,适用于新疆巴旦杏中苦杏仁苷的含量测定,并为其质量控制提供理论依据.
HAN Hai-xia;YANG Xiao-jun;Ayiguli·Aihemaiti;WANG Ying;ZHU Jin-fang;BAO Xiao-wei;MA Juan. Determination of Amygdalin in Xinjiang Amygdalus persica L.by HPLC[J]. .
韩海霞;杨晓君;阿依古丽·艾合买提;王颖;朱金芳;包晓玮;马娟. HPLC法测定新疆巴旦杏中苦杏仁苷的含量[J]. .
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