]*>","")" /> Screening of Transgenic Cotton with an Insect (Microdera puntipennis dzungarica) Antifreezing Protein Gene via Pollen-tube Pathway
Xinjiang Agricultural Sciences

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Screening of Transgenic Cotton with an Insect (Microdera puntipennis dzungarica) Antifreezing Protein Gene via Pollen-tube Pathway

MA Ji;LI Chun-ping;QIU Li-ming;XU Jian-hui;ZHAO Gan;LI Su-li   

  • Received:2008-03-25 Revised:2008-03-25 Online:2008-03-25 Published:2008-03-25

花粉管介导的准噶尔小胸鳖甲抗冻蛋白转基因棉花的筛选

马纪;李春平;邱立明;徐建辉;赵干;李素丽   

  1. 新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐,830046;新疆农科院经作所,乌鲁木齐,830091





摘要： 利用新疆荒漠昆虫抗冻蛋白基因进行遗传转化是培育新疆棉花抗寒品种的新途径. 研究采用RT-PCR结合RACE技术从新疆荒漠昆虫准噶尔小胸鳖甲(Microdera puntip ennis dzungarica)中克隆获得抗冻蛋白(Antifreeze protein)基因Mp AFP149.将MpAFP149克隆至 pBI121上,获得重组质粒后采用电转化法将重组质粒转入农杆菌EHA105内,PCR筛选鉴定重组子,并测序确定抗冻蛋白植物表达载体构建正确.将pBI121-MpAFP通过田间的花粉管导入技术转化棉花,收取转化的棉花种子,经检测证明在转化10 000朵花中,卡那霉素抗性筛选出 13株,PCR检测有2株阳性植株.实验结果为进一步开展抗冻蛋白基因转化棉花的研究奠定了基础.