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WANG Qi-cai;MA Ji;ZHANG Fu-chun
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王起才;马纪;张富春
摘要: 以喀什哈尔葡萄单芽茎段为材料,进行了离体组织培养并以实验获得的喀什哈尔葡萄组培苗进行了外植体器官直接再生研究.结果表明:喀什哈尔葡萄单芽茎段最适初始培养基是:MS+6-BA 1.0 mg/L+NAA 0.01 mg/L,侧芽萌芽率达100;;最适继代培养基是:MS+KT 0.1 mg/L+IBA 0.4 mg/L,同时伸长生长与生根生长,生根率达100;.探讨了喀什哈尔葡萄器官再生的影响因素,并以叶片为试材,建立了植株再生体系.叶片不定芽再生的最佳培养基是MS+6-BA 2.0 mg/L+IBA 0.2 mg/L ,再生率为14.3;;最佳生根培养基是MS+KT 0.1 mg/L+IBA 0.4 mg/L,生根效果良好,生根率为92;.
WANG Qi-cai;MA Ji;ZHANG Fu-chun. Tissue Culture and Establishment of Regeneration System of Kashihare Grape[J]. .
王起才;马纪;张富春. 喀什哈尔葡萄组织培养及再生体系的建立[J]. .
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