]*>","")" /> Cloning, Expression and Purification of gtrA Gene in Sinorhizobium meliloti Rm 1021
Xinjiang Agricultural Sciences

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Cloning, Expression and Purification of gtrA Gene in Sinorhizobium meliloti Rm 1021

YU Ai-yuan;CHEN Ai min;YANG Hai-yan   

  • Received:2008-01-25 Revised:2008-01-25 Online:2008-01-25 Published:2008-01-25

苜蓿中华根瘤菌Rm1021中gtrA基因的克隆、表达及纯化

尉爱远;陈爱民;杨海燕   

  1. 新疆农业大学食品科学学院,乌鲁木齐,830052;新疆兵团康地农业高新技术研究中心,乌鲁木齐,830011





摘要： 对苜蓿中华根瘤菌中GntR家族转录因子基因gtrA进行了表达和纯化,以期进一步研究其功能.以苜蓿根瘤菌Rm1021的基因组为模板,PCR扩增出gtrA基因,并克隆到表达载体PET-28b(+)上.重组质粒经酶切和测序证实正确,然后转化大肠杆菌BL21.'SDS-PAGE显示经IPTG诱导后能得到和理论值大小一致的蛋白条带,通过进一步纯化得到gtrA的表达蛋白.