]*>","")" /> Isolation and Purification of Cecropin-XJ Expressed in Pichia Pastoris by Three Chromatographic Methods
Xinjiang Agricultural Sciences

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Isolation and Purification of Cecropin-XJ Expressed in Pichia Pastoris by Three Chromatographic Methods

DOU Jun;ZHENG Shu-tao;WANG Ping;ZHANG Fu-chun   

  • Received:2007-05-25 Revised:2007-05-25 Online:2007-05-25 Published:2007-05-25

三步层析法分离毕赤酵母工程菌中表达的新疆家蚕抗菌肽

窦君;郑树涛;王娉;张富春   

  1. 新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐,830046





摘要： 通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽.结果表明,三步层析后,SDS电泳显示单一条带,分子量约为7.0 kDa;所纯化的新疆家蚕抗菌肽具有较强的抗菌活性.该纯化工艺的建立,为新疆家蚕抗菌肽在饲料添加剂、农用抗生素等方面的广泛应用奠定基础.