]*>","")" /> Quantitative Analysis Expression of KCR and HCD during Development in High Erucic Acid Bassica Juncea
Xinjiang Agricultural Sciences

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Quantitative Analysis Expression of KCR and HCD during Development in High Erucic Acid Bassica Juncea

MENG Ji-hong;CHEN Yue-hua;LIN Ping;GU Yuan-guo;FAN Ling   

  • Received:2007-04-25 Revised:2007-04-25 Online:2007-04-25 Published:2007-04-25

KCR、HCD基因在高芥酸芥菜型油菜(Brassica Juncea)生育期内的量化表达

孟继红;陈跃华;林萍;顾元国;范玲   

  1. 新疆农科院核生所,乌鲁木齐,830091;新疆农业大学农学院,?诼衬酒?830052;新疆农科院经作所,乌鲁木齐,830091;新疆农科院核生所,乌鲁木齐,830091





摘要： 研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和 QuantumRNA18STM内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析,了解芥酸的合成与功能基因量化表达的关系,为芥菜型油菜的高芥酸品种的选育提供理论依据.实验表明,KCR、HCD基因在高芥酸芥菜型油菜整个生育期中均有表达,即不但在种子发育期表达,在全生育期内叶中也表达,并且显示芥酸合成基因表达量的变化与油菜的发育时期相关.同时也表明具有内标的常规RT-PCR技术可实现对芥酸合成基因的量化表达分析.