]*>","")" /> Study on Purification of β-Mannanase from CXJZ95-198 Strain

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Study on Purification of β-Mannanase from CXJZ95-198 Strain

LI Bao-kun;LIU Zheng-chu;FENG Xiang-yuan;DUAN Sheng-wen;HU Zhen-xiu;PENG Yuan-de;ZHENG Ke   

  • Received:2007-04-25 Revised:2007-04-25 Online:2007-04-25 Published:2007-04-25

CXJZ95-198菌株β-甘露聚糖酶的纯化条件研究

李宝坤;刘正初;冯湘沅;段盛文;胡镇修;彭源德;郑科   

  1. 石河子大学食品学院,新疆石河子,832003;中国农业科学院麻类研究所,长沙,410006

摘要: 研究通过超滤、硫酸铵沉淀、丙酮沉淀及凝胶层析等纯化步骤,将CXJZ95-198菌株所分泌的β-甘露聚糖酶进行了纯化,其纯化倍数为12.05,收率为33.50;.通过SDS-聚丙烯酰胺电泳得到单一条带蛋白,分子量为52.29 kD.