]*>","")" /> Preliminary Study on Gene Clone and Expression About Four Subunits of Islet Activity Protein (IAP) B-oligomer
Xinjiang Agricultural Sciences

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Preliminary Study on Gene Clone and Expression About Four Subunits of Islet Activity Protein (IAP) B-oligomer

PAN Xu-wen   

  • Received:2007-03-25 Revised:2007-03-25 Online:2007-03-25 Published:2007-03-25

胰岛激活蛋白B聚体的各亚基克隆及表达的初步研究

潘煦文   

  1. 新疆农业大学动物营养实验室,乌鲁木齐,830052





摘要： 研究将百日咳杆菌用B-G培养基活化后再在改良的S-S培养基上液体培养,用CTAB-NaCl法提取百日咳杆菌的基因组DNA.经PCR扩增出胰岛激活蛋白 B聚体的四个亚基基因,分别将其克隆于pGEX-6p-1载体上,并探讨了在大肠杆菌BL21(DE3)中可溶性表达IAP B聚体各亚基的可能性.通过控制诱导表达温度,S2,S3均可以实现可溶表达,以GST亲和层析得到的S2终产物0.4 mg/L培养物, S3为0.6 mg/L培养物.S4表达虽是可溶性的,但由于融合蛋白的等电点近于7,多步纯化易损失.S5的表达量太低,有待进一步分析.