]*>","")" /> The Establishment and Optimization of Tissue Culture System of Sinningia speciosa

• Article • Previous Articles     Next Articles

The Establishment and Optimization of Tissue Culture System of Sinningia speciosa

QIN Li;HU XUE-mei;LIAO Qing;GAO Yan;SHA Hong;Duolikun·Shabire;HE Bin;ZHANG Fu-chun   

  • Received:2007-03-25 Revised:2007-03-25 Online:2007-03-25 Published:2007-03-25

观赏植物大岩桐(Sinningia speciosa)组织培养体系的建立和优化

秦丽;胡雪梅;廖青;高燕;沙红;多里坤·沙比尔;贺宾;张富春   

  1. 新疆生物资源基因工程重点实验室,乌鲁木齐,830046;新疆农科院经作所,乌鲁木齐,830091;新疆农科院经作所,乌鲁木齐,830091;新疆生物资源基因工程重点实验室,乌鲁木齐,830046

摘要: 实验探索了热带观赏植物大岩桐离体快繁的有效途径,以大岩桐叶片作为外植体,建立再生体系.各阶段的最佳培养基配比结果如下:初代培养基:MS+6-BA 1.0 mg/L+NAA 0.5 mg/L;增殖培养基:MS+6-BA 1.0 mg/L + NAA 0.05 mg/L;壮苗培养基:MS+6-BA 1.0 mg/L+NAA 0.8 mg/L;生根培养基:1/2MS,得到的组培苗经炼苗移栽后成活率高达100;.该体系的建立为其规模化生产及遗传改良提供了前提条件.