]*>","")" /> Screening for High Virulent Mutant Strain of Metarhizium Anisopliae
Xinjiang Agricultural Sciences

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Screening for High Virulent Mutant Strain of Metarhizium Anisopliae

  • Received:2004-05-25 Revised:2004-05-25 Online:2004-05-25 Published:2004-05-25

绿僵菌高毒力菌株的选育

施宠;付建红;王纯利   

  1. 新疆农业大学资源与环境学院,新疆乌鲁木齐,830052;新疆农科院微生物所,新疆乌鲁木齐,830000





摘要： 以金龟子绿僵菌为出发菌株,经物理、化学诱变,确定诱变条件.经诱变初筛时,采用在诱导培养基上测量单菌落大小及透明圈大小的方法,计算其比值,保留比值较大的菌株.再通过复筛测菌株弹性凝乳蛋白酶(Pr1)酶活获得一诱变菌株M6.该突变株产生Pr1活性比出发菌株提高1.10倍,在整个诱变过程中,以出发菌株,代号M原为对照.