]*>","")" /> Improved of Total DNA Extraction Approaches in Upland Cotton and Polymorphic Loci Amplified by SSR

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Improved of Total DNA Extraction Approaches in Upland Cotton and Polymorphic Loci Amplified by SSR

  • Received:2004-03-25 Revised:2004-03-25 Online:2004-03-25 Published:2004-03-25

棉花DNA提取方法的优化及SSR标记在多态筛选的应用

王沛政;胡保民   

  1. 新疆康地农业科技发展有限公司,新疆乌鲁木齐,830011

摘要: 对棉花DNA的提取方法进行了优化,通过改良方法所提取的棉花总DNA的质量得以提高.为了进一步证实改良的总DNA提取方法的效果,对所构建的抗黄萎病QTL定位群体的双亲进行了SSR-PCR检测,得到了清晰的多态性SSR标记,为研究分子标记在棉花育种的应用打下了基础.