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JIAO Zi-wei;ZHANG Xiang-feng;ZHANG Na;Wueren;GUO Yan-bin
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焦子伟;张相锋;张娜;吾尔恩;郭岩彬
摘要: [目的]水生拉恩氏菌(Rahnella aquatilis)HX2能合成葡萄糖脱氢酶(glucose dehydrogenase,GDH)和吡咯喹啉醌(pyrroloquinoline quinone,PQQ),PQQ为GDH辅酶,与其共同参与葡萄糖溶磷代谢.揭示不同碳源条件下PQQ影响HX2菌株溶解无机磷作用的机理.[方法]以野生菌株HX2、突变体MH15及其互补菌株CMH15(pqq)为供试材料,在不同碳源条件下采用平板溶磷、钼锑抗比色法等方法对其进行溶解无机磷定性、定量以及pH相关分析.[结果]除D-山犁醇、D-果糖外,PQQ参与HX2菌株对木糖、葡萄糖、D-甘露糖、D-甘露醇、蔗糖、乳糖6种碳源的溶磷代谢,但其溶磷代谢因HX2菌株利用不同碳源的能力而不同,以乳糖利用最低,木糖利用最高.[结论]PQQ作为GDH的辅酶,两者共同参与HX2菌株的木糖、葡萄糖、D-甘露糖、D-甘露醇、蔗糖和乳糖溶解无机磷代谢,并起到重要调控作用.
JIAO Zi-wei;ZHANG Xiang-feng;ZHANG Na;Wueren;GUO Yan-bin. Influences of Pyrroloquinoline Quinone on Inorganic Phosphate Solubilization under Different Carbon Sources in Rahnella aquatilis HX2[J]. .
焦子伟;张相锋;张娜;吾尔恩;郭岩彬. 不同碳源条件下PQQ对植物促生菌Rahnella aquatilis HX2溶解无机磷影响的研究[J]. .
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http://www.xjnykx.com/EN/Y2016/V53/I2/295