]*>","")" /> Rapid PCR Detection Technology for Four Species of Valsa Canker Pathogens of Trees in Xinjiang
Xinjiang Agricultural Sciences

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Rapid PCR Detection Technology for Four Species of Valsa Canker Pathogens of Trees in Xinjiang

GUO Kai-fa;YAO Zhao-qun;WU Cai-lan;XIANG Ben-chun;ZHAO Si-feng   

  • Received:2016-06-25 Revised:2016-06-25 Online:2016-06-25 Published:2016-06-25

新疆4种林木腐烂病菌PCR快速检测技术研究

郭开发;姚兆群;吴彩兰;向本春;赵思峰   

  1. 新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室/石河子大学农学院,新疆石河子,832003

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摘要： [目的]建立新疆林木腐烂病菌的快速PCR检测方法,为新疆林木腐烂病的早期测报和防治提供技术依据.[方法]针对新疆林木腐烂病菌主要种苹果黑腐皮壳(Valsa mali,污黑腐皮壳(Valsa.sordida),Leucostoma niveu和Valsa.malicola的rDNA-ITS特异性区段设计属专化型引物和种特异性引物.[结果]属专化型引物VF/VR可以从腐烂病菌中扩增一条424 bp的条带,检测灵敏度为10 pg/mL;设计的4对种特异性引物分别可对4种腐烂病菌V.mali,V.sordida,L.nivecum,V.malicola检测到263 bp、423 bp、307 bp、308bp的条带,检测灵敏度均为10 pg/mL.[结论]采用腐烂病菌Valsa的rDNA-ITS特异性区段设计的引物及PCR方法,可用于新疆林木腐烂病的快速分子检测.