]*>","")" /> The Induction of Hydrogen Peroxide on Resveratrol in Detached Grape Leaves
Xinjiang Agricultural Sciences

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The Induction of Hydrogen Peroxide on Resveratrol in Detached Grape Leaves

HUANG Fang-ai;ZHANG Bo;YANG Xiao-yan;YAN Huan;CHEN Han-ying;ZHENG Qiu-sheng   

  • Received:2012-06-25 Revised:2012-06-25 Online:2012-06-25 Published:2012-06-25

H2O2对葡萄离体叶片白藜芦醇的诱导作用

黄方爱;张波;杨晓燕;颜欢;陈韩英;郑秋生   

  1. 石河子大学药学院,新疆石河子,832002;省部共建新疆特种资源植物药重点实验室,新疆石河子,832002





摘要： [目的]葡萄叶片白藜芦醇含量较虎杖根含量低,难以作为药用提取用途,研究采用诱导剂升高葡萄离体叶片中白藜芦醇的含量.[方法]实验采用H2O2对离体红地球葡萄叶片进行诱导,采用薄层及高效液相色谱法分析白藜芦醇的含量变化,并使用RT - PCR方法分析茋合酶基因(STS)的表达量变化.[结果]H2O2对离体叶片茋类物质具有显著的诱导作用,诱导白藜芦醇的积累呈现显著的量时依赖性；5;H2O2处理48 h后叶片白藜芦醇含量可提高15倍以上,鲜重达到263 μg/g.[结论]H2O2可以作为葡萄离体叶片白藜芦醇的有效诱导剂.